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Merck
CN

CS0740

Acid phosphatase Assay Kit

1 kit sufficient for 1,000 assays (multiwell plates), 1 kit sufficient for 100 assays (tubes)

Synonym(s):

Acid Phosphatase Enzyme Activity Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
eCl@ss:
42010102
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Quality Level

usage

 kit sufficient for 1,000 assays (multiwell plates),  kit sufficient for 100 assays (tubes)

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... ACP1(52), ACP2(53)

Application

The kit is used for the detection of acid phosphatase activity in tissues, whole cell extracts, column fractions and purified enzyme. The kit contains all the reagents required for a fast and simple acid phosphatase detection. The kit contains a standard solution and a control enzyme.

Biochem/physiol Actions

Acid phosphatase is one of the acid hydrolases that normally reside in lysosomes. It is a marker for the identification of lysosomes in sub-cellular fractionations.


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Kit Components Only

Product No.
Description

  • p-Nitrophenyl phosphate tablets 5 mg 20 tablets

  • Citrate buffer solution, 0.09 M pH 4.8 100 mL

  • p-Nitrophenol Standard solution 10 mM 1 mL

  • Acid phosphatase control enzyme .2 mL

pictograms

Health hazard

signalword

Warning

hcodes

Hazard Classifications

Carc. 2 - STOT RE 2 Oral

target_organs

Liver,Kidney

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3

Regulatory Information

低风险生物材料
易制毒化学品(2类)

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Articles

亚细胞组分离心分离是一种常用技术,广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同,因此通过离心,可从温和均质的样品中轻松分离和纯化细胞器组分。

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.


Hua Zhao et al.
Journal of neuroscience methods, 217(1-2), 67-74 (2013-04-24)
Cobalamin (Cbl) utilization as a cofactor for methionine synthase and methylmalonyl-CoA mutase is dependent on the transport of Cbl through lysosomes and its subsequent delivery to the cytosol and mitochondria. We speculated that neuropathological conditions that impair lysosomal function (e.g.
Rabea Asleh et al.
The Journal of biological chemistry, 289(23), 16313-16325 (2014-04-30)
The major function of the Haptoglobin (Hp) protein is to control trafficking of extracorpuscular hemoglobin (Hb) thru the macrophage CD163 receptor with degradation of the Hb in the lysosome. There is a common copy number polymorphism in the Hp gene
Xiaoting Chen et al.
Journal of cellular and molecular medicine, 23(6), 4290-4300 (2019-04-12)
Impaired autophagic degradation of intracellular lipids is causally linked to the development of non-alcoholic steatohepatitis (NASH). Pharmacological agents that can restore hepatic autophagic flux could therefore have therapeutic potentials for this increasingly prevalent disease. Herein, we investigated the effects of



Global Trade Item Number

SKUGTIN
CS0740-1KT04061833553121