D0422
DMEM - high glucose
With sodium bicarbonate, without ʟ-methionine, ʟ-cystine and ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
Product Name
Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose and sodium bicarbonate, without L-methionine, L-cystine and L-glutamine, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
glucose: high
phenol red: yes
L-glutamine: no
sodium pyruvate: yes
shipped in
ambient
storage temp.
2-8°C
Application
Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".
Preparation Note
Supplement with 0.584 g/L L-glutamine.
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signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Instructions
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Methionine (Met) is an essential amino acid and critical precursor to the cellular methyl donor S-adenosylmethionine. Unlike nontransformed cells, cancer cells have a unique metabolic requirement for Met and are unable to proliferate in growth media where Met is replaced
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ATP depletion inhibits cell cycle progression, especially during the G1 phase and the G2 to M transition. However, the effect of ATP depletion on mitotic progression remains unclear. We observed that the reduction of ATP after prometaphase by simultaneous treatment
Global Trade Item Number
| SKU | GTIN |
|---|---|
| D0422-100ML | 04061833557662 |