D1152
Dulbecco′s Modified Eagle′s Medium - high glucose
HEPES Modification, With 4500 mg/L glucose, L-glutamine, and 25 mM HEPES, without sodium bicarbonate and pyruvate, powder, suitable for cell culture
Synonym(s):
DME, DMEM
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
NACRES:
NA.75
UNSPSC Code:
12352207
form
powder
technique(s)
cell culture | mammalian: suitable
components
sodium pyruvate: no
NaHCO3: no
phenol red: yes
HEPES: 25 mM
L-glutamine: yes
glucose: high
shipped in
ambient
storage temp.
2-8°C
Application
Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".
Preparation Note
Formulated to contain 17.3 grams of powder per liter of medium.
Supplement with 3.7 g/L sodium bicarbonate.
Still not finding the right product?
Explore all of our products under Dulbecco′s Modified Eagle′s Medium - high glucose
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Protocols
粉末培养基及盐混合物极具吸湿性，应注意防潮。
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
Related Content
Instructions
Farlen J B Miranda et al.
Poultry science, 95(1), 12-18 (2016-01-23)
Raising ostriches became an important economic activity after their products became commodities. The health of farm animals is of paramount importance, so assessing basic immunological responses is necessary to better understand health problems. We developed a method to obtain ostrich
Kiran Pandey et al.
Animal reproduction science, 181, 93-102 (2017-04-24)
Gonadotropin-releasing hormone receptors (GnRHRs) colocalize with insulin and glucocorticoid receptors in lipid rafts of the gonadotroph plasma membrane, where they facilitate downstream signaling. We recently found that orphan G-protein-coupled receptor (GPR)61 is expressed in the anterior pituitary (AP) of heifers
Kiran Pandey et al.
Animal science journal = Nihon chikusan Gakkaiho, 89(1), 60-71 (2017-09-30)
We recently found that orphan G-protein-coupled receptor (GPR)153 is expressed in the anterior pituitary (AP) of heifers, leading us to speculate that GPR153 colocalizes with gonadotropin-releasing hormone receptor (GnRHR) in the plasma membrane of gonadotrophs and is expressed at specific
Global Trade Item Number
| SKU | GTIN |
|---|---|
| D1152-10L | 04061838259226 |
| D1152-50K | 04061834180180 |
| D1152-25KG | 04061834408260 |
| D1152-50KG | 04061834408277 |
| D1152-10X1L | 04061838259233 |
| D1152-50L | 04061833558775 |