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D1556

OptiPrep™ Density Gradient Medium

used for cell and subcellular organelle isolation

Synonym(s):

Iodixanol solution

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About This Item

CAS Number:
92339-11-2
NACRES:
NA.75
PubChem Substance ID:
329798703
UNSPSC Code:
12352207
MDL number:
MFCD00867965
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Quality Level

200

sterility

sterile

form

liquid

density

1.319-1.321 g/mL

application(s)

hematology
histology

storage temp.

room temp

SMILES string

CC(=O)N(CC(O)CN(C(C)=O)c1c(I)c(C(=O)NCC(O)CO)c(I)c(C(=O)NCC(O)CO)c1I)c2c(I)c(C(=O)NCC(O)CO)c(I)c(C(=O)NCC(O)CO)c2I

InChI

1S/C35H44I6N6O15/c1-13(52)46(30-26(38)20(32(59)42-3-15(54)9-48)24(36)21(27(30)39)33(60)43-4-16(55)10-49)7-19(58)8-47(14(2)53)31-28(40)22(34(61)44-5-17(56)11-50)25(37)23(29(31)41)35(62)45-6-18(57)12-51/h15-19,48-51,54-58H,3-12H2,1-2H3,(H,42,59)(H,43,60)(H,44,61)(H,45,62)

InChI key

NBQNWMBBSKPBAY-UHFFFAOYSA-N

General description

OptiPrep™ Density Gradient Medium, also known as iodixanol, is a low osmolarity, non-ionic iodinated density gradient medium. It is a sterile and endotoxin-tested solution that comprises 60% (w/v) iodixanol in water, designed for the in vitro isolation of biological particles. It may be used for fractionation and separation of cell and subcellular organelles.

Application

OptiPrep™ has been used to isolate molecules, cells, and subcellular organelles, including DNA, cytokines, lysosomes, endoplasmic reticulum, peroxisomes, T lymphocytes, and natural killer cells. It has also been used for the following applications:

  • to investigate the efficiency of single-cell encapsulation.
  • the gradient-based isolation of senescent cancer cells.
  • isolation of non-enveloped virion isolation from bronchoalveolar lavage.
  • isolation of lipoproteins and macromolecules
  • purification of virions
  • purification of neuron cells

Features and Benefits

OptiPrep™ has numerous biocompatible properties that make it ideal for use.
  • It is biologically inert and does not interfere with assays for marker enzymes.
  • It is non-ionic, non-toxic, and has low osmotic pressure.
  • It forms spontaneous gradients that are stabler and easier to prepare than sucrose.

Other Notes

60% (w/v) solution of iodixanol in water (sterile).

Legal Information

OptiPrep is a trademark of Serumwerk Bernburg AG

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCH1628
MKCQ8495
MKCQ8494
BCCG2789
BCCG3620

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Articles

Centrifugation Basics

Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.

WU Polyomavirus in Respiratory Epithelial Cells from Lung Transplant Patient with Job Syndrome
Erica A. S, et al.
Emerging Infectious Diseases, 21(1), 103-106 (2015)
Tobias Boothe et al.
eLife, 6 (2017-07-15)
In light microscopy, refractive index mismatches between media and sample cause spherical aberrations that often limit penetration depth and resolution. Optical clearing techniques can alleviate these mismatches, but they are so far limited to fixed samples. We present Iodixanol as
Tanner C Fadero et al.
eLife, 6 (2017-09-05)
Iodixanol provides an easy and affordable solution to a problem that has limited resolution and brightness when imaging living samples.
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Global Trade Item Number

SKUGTIN
D1556-100ML04061837280368
D1556-250ML04061838261700