D2001
Deoxyribonucleic acid sodium salt from Escherichia coli strain B
Type VIII
Synonym(s):
DNA
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About This Item
CAS Number:
UNSPSC Code:
41106305
eCl@ss:
32160414
EC Number:
297-226-7
NACRES:
NA.51
MDL number:
Form:
fibers
Solubility:
water: 0.98-1.02 mg/mL, faintly hazy to hazy, colorless to light yellow
Storage temp.:
2-8°C
type
Type VIII
Quality Level
form
fibers
impurities
<2% protein
color
, white to white with yellow cast
solubility
water: 0.98-1.02 mg/mL, faintly hazy to hazy, colorless to light yellow
λmax
256-264 nm (15mM NaCl+1.5mM Trisodium Citrate PH7)
storage temp.
2-8°C
Application
Deoxyribonucleic acid sodium salt from Escherichia coli strain B has been used to measure the melting temperature (Tm) and in differential scanning calorimetry (DSC) studies. It has also been used as a template sequence in the polymerase chain reaction (PCR).
Escherichia coli DNA has many uses in research. Escherichia coli DNA (ecDNA) is used in studies of DNA binding agents that modulate DNA structure and function. E. coli DNA is used in physicochemical studies of DNA behavior in solution, and to evaluate species-specific DNA structures and binding domains. This DNA is particularly useful for genomic analysis, including PCR, library construction in bacteriophage lambda, and in heterologous pre-hybridization and hybridization protocols.
Preparation Note
Prepared by procedure of Marmur, J., J. Mol. Biol., 3, 208 (1961).
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
常规特殊物品
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R Richins et al.
Biotechnology progress, 17(2), 252-257 (2001-04-21)
The Escherichia coli DNA binding protein FIS is a transcriptional modulator involved in the regulation of many cellular processes, including the activation of rRNA synthesis. High-level overproduction of FIS in early, mid, or late log cultures resulted in growth-phase- and
Differential scanning calorimetry of bacteria
Miles CA, et al.
Microbiology, 132(4), 939-952 (1986)
Mitsumasa Hashimoto et al.
The Journal of biological chemistry, 278(31), 28501-28507 (2003-05-16)
Closely opposed lesions form a unique class of DNA damage that is generated by ionizing radiation. Improper repair of closely opposed lesions could lead to the formation of double strand breaks that can result in increased lethality and mutagenesis. In
Global Trade Item Number
| SKU | GTIN |
|---|---|
| D2001-10MG | 04061833561263 |
| D2001-25MG | 04061833289358 |
| D2001-50MG | 04061833289365 |
| D2001-5MG | 04061833289372 |