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D4293

3,3′-Diaminobenzidine

SIGMAFAST™, chromogenic, tablet

Synonym(s):

DAB tablets

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About This Item

CAS Number:
91-95-2
UNSPSC Code:
12352204
PubChem Substance ID:
24893856
NACRES:
NA.83
MDL number:
MFCD00007725
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Product Name

SIGMAFAST™ 3,3′-Diaminobenzidine tablets, tablet, To prepare 5 mL

Quality Level

200

form

tablet

storage temp.

−20°C

SMILES string

Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2

InChI key

HSTOKWSFWGCZMH-UHFFFAOYSA-N

Application

SIGMAFAST™ 3,3′-Diaminobenzidine tablets has been used as a chromogen for visualization in immunohistochemistry of gonad tissue, thymus, dental chronic periapical lesions and gall bladder sections.
Developed for use in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. DAB is the immunohistology substrate of choice as it produces an intense brown-black stain which is resistant to alcohol. Slides stained with DAB may be dehydrated, mounted in resinous media, and stored for future reference. SIGMAFAST™ DAB Tablets require no additional buffers or steps to prepare an active substrate solution. Tablets are individually packaged in foil packets

Preparation Note

Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing DAB and urea hydrogen peroxide.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

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Danger

Hazard Classifications

Carc. 1B - Eye Dam. 1 - Muta. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3

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Certificates of Analysis (COA)

Lot/Batch Number
SLCG5357
SLCD1662
SLBX4976
SLBR2973V
SLBM5773V

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Articles

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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Global Trade Item Number

SKUGTIN
D4293-50SET04061835506231
D4293-5SET04061833564271
D4293-5X50SET04061833629604