D5523
Dulbecco′s Modified Eagle′s Medium - low glucose
With 1000 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
DME, DMEM
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About This Item
Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
L-glutamine: yes
NaHCO3: no
sodium pyruvate: yes
phenol red: yes
HEPES: no
glucose: low
shipped in
ambient
storage temp.
2-8°C
General description
Dulbecco′s Modified Eagle′s medium (DME) is a modification of Basal Medium Eagle (BME) that comprises a four-fold higher concentration of amino acids and vitamins, as well as additional supplementary components. The original DME formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types.
Application
Dulbecco′s Modified Eagle′s medium-low glucose has been used in the differentiation and culture of human and murine mesenchymal stem cells (MSCs).
Preparation Note
Formulated to contain 10.0 grams of powder per liter of medium.
Supplement with 3.7 g/L sodium bicarbonate.
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signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Sens. 1
Storage Class
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
粉末培养基及盐混合物极具吸湿性，应注意防潮。
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
Related Content
Instructions
Daniela Steffens et al.
Journal of biomaterials applications, 32(5), 663-676 (2017-10-11)
Laminin-functionalized poly-d,l-lactic acid scaffolds were produced. Following this, mesenchymal stem cells and keratinocytes were seeded on biomaterials for the in vivo experiments, where the biomaterials with or without cells were implanted. The analysis is comprised of the visual aspect and mean
Hsi-Hsien Hsu et al.
Journal of cellular physiology, 233(7), 5458-5467 (2017-12-17)
Oxaliplatin (OXA), is a third generation platinum drug used as first-line chemotherapy in colorectal cancer (CRC). Cancer cells acquires resistance to anti-cancer drug and develops resistance. ATP-binding cassette (ABC) drug transporter ABCG2, one of multidrug resistance (MDR) protein which can
Gayatri Jamwal et al.
Biochimica et biophysica acta, 1865(6), 920-931 (2018-04-06)
IGF1R is a ubiquitous receptor tyrosine kinase that plays critical roles in cell proliferation, growth and survival. Clinical studies have demonstrated upregulation of IGF1R mediated signaling in a number of malignancies including colon, breast, and lung cancers. Overexpression of the
Global Trade Item Number
| SKU | GTIN |
|---|---|
| D5523-25KG | 04061834408352 |
| D5523-10L | 04061833586358 |
| D5523-10X1L | 04061835522576 |
| D5523-50KG | 04061834408369 |
| D5523-50K | 04061834180289 |
| D5523-50L | 04061833586365 |