D5652
Dulbecco′s Phosphate Buffered Saline
Modified, without calcium chloride and magnesium chloride, powder, suitable for cell culture
Synonym(s):
DPBS
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About This Item
Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
pH
7.2-7.8
solubility
water: 9.6 g/L, clear
storage temp.
2-8°C
General description
Dulbecco′s Phosphate Buffered Saline is a balanced salt solution (BSS) widely used in tissue culture.
Application
Dulbecco′s Phosphate Buffered Saline has been used:
- as one of the pseudo-physiological solutions to study the in vitro degradation behavior of Fe–20Mn–1.2C alloy
- to dissolve Angelica gigas powder for microencapsulation
- to wash cells as part of cell culture treatments, wound-healing assay, transwell assay and apoptosis assay
- to wash amniotic membrane for cellular analyses
D-PBS is used to wash cells during preparation and serial transfer.
Biochem/physiol Actions
Dulbecco′s Phosphate Buffered Saline serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance. It hydrates cells and acts as a source of bulk inorganic ions essential for normal cell metabolism. This buffered saline acts as the principal energy source for cell metabolism when combined with a carbohydrate, such as glucose. It provides a buffering system to maintain the medium within the physiological pH range.
Preparation Note
Formulated to contain 9.6 grams of powder per liter of medium.
Other Notes
This D-PBS is formulated without Ca or Mg. Formulations with Ca and Mg added are also available
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Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| D5652-10L | 04061838259318 |
| D5652-200L | 04061834170662 |
| D5652-10X1L | 04061835514106 |
| D5652-1L | 04061837468315 |
| D5652-2000L | 04061837469190 |
| D5652-50L | 04061835518968 |