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D5751

Blue Dextran

Void volume marker for gel filtration columns.

Synonym(s):

Dextran blue from Leuconostoc mesenteroides

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About This Item

CAS Number:
87915-38-6
UNSPSC Code:
12352200
NACRES:
NA.25
MDL number:
MFCD00130661
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biological source

non-animal source

Quality Level

200

form

powder

quality

200

mol wt

2,000,000

technique(s)

affinity chromatography: suitable

suitability

suitable for GFC marker

General description

Blue Dextran is a soluble derivative of dextran. Its structure includes triazinyl dye Cibacron blue F3GA covalently bound to high molecular weight (2000 kDa) dextran. Blue Dextran is generally used as a marker to determine void volume in gel filtration chromatography columns.

Application

Blue Dextran has been used for calibration of size exclusion chromatography (SEC) column. It has also been used in Dextran blue permeability assays to determine the integrity of co-cultures containing epithelial cells. Blue dextran may be used in affinity chromatography, gel filtration chromatography, protein chromatography, and molecular weight markers. Blue dextran is also suitable to study Alzheimer′s disease.

Preparation Note

Prepared from dextran with an average mol. wt. of 2,000,000.

Analysis Note

degree of substitution: 0.10 to 0.12 mmol Cibracon Blue per gram of dextran

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SLCG5633
SLBW8211
SLBP3949V
SLBH8366V
SLBB1849V

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Protocols

HPLC Analysis of Glycans

Uncover how HPLC analysis of glycans helps researchers analyze glycan structures and glycoforms & find products to assist in your glycan analysis methods.

Osao Adachi et al.
Bioscience, biotechnology, and biochemistry, 74(12), 2438-2444 (2010-12-15)
The membrane fraction of Gluconobacter oxydans IFO 3244, involving membrane-bound quinoprotein quinate dehydrogenase and 3-dehydroquinate dehydratase, was immobilized into Ca-alginate beads. The Ca-alginate-immobilized bacterial membrane catalyzed a sequential reaction of quinate oxidation to 3-dehydroquinate and its spontaneous conversion to 3-dehydroshikimate
Catherine Chiu et al.
Fluids and barriers of the CNS, 9(1), 3-3 (2012-01-25)
Amyloid accumulation in the brain parenchyma is a hallmark of Alzheimer's disease (AD) and is seen in normal aging. Alterations in cerebrospinal fluid (CSF) dynamics are also associated with normal aging and AD. This study analyzed CSF volume, production and
Liguo Zhang et al.
Journal of colloid and interface science, 368(1), 648-654 (2011-12-27)
The objective is to investigate the influence of sonication on the mechanical and release properties of hydrogel capsules. A new fabrication process is developed to fabricate millimetric capsules made of a highly-viscous liquid core protected by a thin hyperelastic alginate

Global Trade Item Number

SKUGTIN
D5751-5G04061833586754
D5751-10G04061833586730
D5751-1G04061833586747