D5921
DMEM - low glucose
With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM
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About This Item
Product Name
Dulbecco′s Modified Eagle′s Medium - low glucose, With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
phenol red: no
sodium pyruvate: no
glucose: low
HEPES: no
L-glutamine: no
NaHCO3: yes
shipped in
ambient
storage temp.
2-8°C
General description
Dulbecco′s Modified Eagle′s medium (DME) is a modification of Basal Medium Eagle (BME), which has a four-fold higher concentration of amino acids and vitamins along with additional supplementary components. The first original DME formula was reported to culture embryonic mouse cells comprising 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types. DME has been shown to improve the stability of the medium.
Application
Dulbecco′s Modified Eagle′s medium-low glucose has been used to culture:
- living colon cancer SW620 cells
- human breast carcinoma cell lines MCF-7(13), KPL and MDA-MB-231
- mammal and canine derived adipose-derived mesenchymal stem cells
Preparation Note
Supplement with 0.584 g/L L-glutamine.
Other Notes
Investigators who work with stem cells or cell clones frequently prefer media without phenol red.
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signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| D5921-500ML | 04061833587065 |
| D5921-6X500ML | 04061833587072 |