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Merck
CN

D8037

Driselase Basidiomycetes sp.

BioReagent, suitable for plant cell culture

Synonym(s):

Driselase from Basidiomycetes sp.

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About This Item

CAS Number:
UNSPSC Code:
10171502
EC Number:
286-055-3
NACRES:
NA.72
MDL number:
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product line

BioReagent

Quality Level

form

powder

composition

Protein, ≥10% biuret

technique(s)

cell culture | plant: suitable

application(s)

agriculture

storage temp.

−20°C

Application

Driselase Basidiomycetes sp. has been used:

  • in spheroplast preparation from Coccomyxa cells
  • in a CRISPR/Cas9-based mutagenesis protocol for Brachypodium distachyon and its allopolyploid relative, Brachypodium hybridum
  • for cell wall digestion to perform whole-mount immunolocalization of Lotus japonicus root tissue

Biochem/physiol Actions

Driselase is a natural mixture of enzyme activities (fungal carbohydrates) used to digest plant cell walls to facilitate the maceration of plant materials, protoplast formation, and extraction processes. Driselase releases cell wall carbohydrates. This formulation contains enzyme activities of cellulose, endo-1,3-β-glucanase, and xylanase.

Other Notes

Crude powder containing laminarinase, xylanase and cellulase.

Legal Information

Driselase is a trademark of ASKA Animal Health Co. Ltd.


pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

常规特殊物品

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G J McDougall et al.
Carbohydrate research, 219, 123-132 (1991-10-14)
The retention times of 10 oligosaccharides, generated from the xyloglucans of Rosa and Tropaeolum by the action of Trichoderma cellulase, and of 17 related carbohydrates, in h.p.l.c. on an amino-substituted silica (Amino-Spheri-5) depended largely on the number of hydroxyl groups
Laura A Moody et al.
The New phytologist, 218(3), 1270-1277 (2018-03-03)
Forward genetics is now straightforward in the moss Physcomitrella patens, and large mutant populations can be screened relatively easily. However, perturbation of development before the formation of gametes currently leaves no route to gene discovery. Somatic hybridization has previously been
D T Kaplan et al.
Journal of nematology, 22(3), 399-406 (1990-07-01)
Radopholus spp. were reared in carrot tissue culture via established procedures, with slight modification. Several plant tissue maceration enzymes and flotation media (salts and sucrose) were evaluated with regard to nematode toxicity and extraction efficiency. Best extraction of viable nematodes



Global Trade Item Number

SKUGTIN
D8037-1G04061833589120
D8037-5G04061833589137