D8273
Deoxyribonucleic acid−cellulose single-stranded from calf thymus DNA
lyophilized powder
Synonym(s):
single-stranded DNA-Cellulose, ss-DNA-Cellulose, DNA-Cellulose
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About This Item
Biological source:
bovine (calf) thymus
Form:
lyophilized powder
Storage temp.:
−20°C
biological source
bovine (calf) thymus
Quality Level
form
lyophilized powder
extent of labeling
≥3 mg single-stranded calf thymus DNA/g
storage temp.
−20°C
General description
Deoxyribonucleic acid−cellulose single-stranded from calf thymus DNA, also known as ctDNA is a natural source of DNA designed for DNA binding agent studies. The detection limits of ctDNA are around 2.0ng and linear ranges of light scattering intensity (I(LS)) is 0.05-1.5 mµg.
Application
Deoxyribonucleic acid−cellulose single-stranded from calf thymus DNA is used to study the binding parameters and binding specificity of a variety of proteins, nucleotides, and chemical reagents including anti-cancer agents. It is frequently used in pull-down assays and chromatographic procedures.
Analysis Note
May contain a trace of Tris and EDTA.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
低风险生物材料
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A Georgaki et al.
Nucleic acids research, 22(7), 1128-1134 (1994-04-11)
A DNA helicase from calf thymus, called DNA helicase F, copurified with replication protein A through several steps of purification including DEAE-Sephacel, hydroxyapatite and single stranded DNA cellulose. It is finally separated from replication protein A on FPLC Mono Q
S S Zhang et al.
The Journal of biological chemistry, 266(30), 20483-20490 (1991-10-25)
Two DNA helicases from calf thymus nuclei have been purified and characterized. The two proteins, designated as nuclear DNA helicase I and II, were copurified on Bio-Rex 70, DEAE-Sepharose, phosphocellulose and subsequently separated from each other on a heparin-Sepharose column.
T Kuzuhara et al.
Biochemical and biophysical research communications, 362(4), 805-810 (2007-09-04)
Tumor necrosis factor-alpha (TNF-alpha) inducing protein (Tipalpha) is a carcinogenic factor secreted from Helicobacter pylori (H. pylori), mediated through both enhanced expression of TNF-alpha and chemokine genes and activation of nuclear factor-kappaB. Since Tipalpha enters gastric cancer cells, the Tipalpha
Global Trade Item Number
| SKU | GTIN |
|---|---|
| D8273-5G | 04061833662755 |
| D8273-10G | 04061833589342 |
| D8273-1G | 04061826690659 |