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Merck
CN

E7510

Earle′s Balanced Salt Solution 10x

Without sodium bicarbonate, 10 ×, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Earle’s Balanced Salts, EBSS

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
Technical Service
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Quality Level

sterility

sterile-filtered

form

liquid

concentration

10 ×

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

glucose: 10.0 g/L
NaHCO3: no
phenol red: 0.11 g/L

shipped in

ambient

storage temp.

room temp

General description

Earle′s Balanced Salt Solution 10x is a saline solution with physiological pH. It is useful in inducing autophagy and promotes Bacillus Calmette-Guérin (BCG)-induced dendritic cells (DCs) maturation.

Application

Earle′s Balanced Salt Solution 10x has been used:
  • as an additive to the nutritive medium sheep nematode Trichostrongylus colubriformis eggs
  • in the swim-up method to prepare human sperm sample
  • in the steady-state medium to culture bovine primary mammary epithelial cells

Features and Benefits

  • Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance.
  • Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism.
  • When combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism.
  • Provides a buffering system to maintain the medium within the physiological pH range (7.2-7.6).

Preparation Note

Supplement with 2.2 g/L sodium bicarbonate at 1×.


Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Yanli Ren et al.
Cancer letters, 390, 126-136 (2017-01-22)
Dyregulation of autophagy is implicated in human cancers and the mechanism details remains largely unclear. Herein we report the regulatory role of miR-638 in autophagy of esophageal squamous cell carcinoma (ESCC) and breast cancer cells. We found that miR-638 overexpression
Luchao Li et al.
Cell death & disease, 12(2), 220-220 (2021-02-28)
Receptor tyrosine kinase (RTK) inhibitors, such as sunitinib and sorafenib, remain the first-line drugs for the treatment of mRCC. Acquired drug resistance and metastasis are the main causes of treatment failure. However, in the case of metastasis Renal Cell Cancer
Zhenlu Li et al.
Cell death & disease, 9(5), 483-483 (2018-05-01)
Autophagy is an essential cytoprotective response against pathologic stresses that selectively degrades damaged cellular components. Impaired autophagy contributes to organ injury in multiple diseases, including ischemia/reperfusion (I/R), but the exact mechanism by which impaired autophagy is regulated remains unclear. Several



Global Trade Item Number

SKUGTIN
E7510-500ML04061833605103
E7510-100ML04061833605097
E7510-6X500ML04061833605158