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EMS0001

PNGase Fast

recombinant, expressed in E. coli

Synonym(s):

N-Glycosidase F, PNGase F, Peptide N-glycosidase

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About This Item

UNSPSC Code:
41131616
Form:
ready-to-use solution
Recombinant:
expressed in E. coli
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recombinant

expressed in E. coli

Quality Level

200

conjugate

(N-linked)

grade

Proteomics Grade

form

ready-to-use solution

shipped in

wet ice

storage temp.

2-8°C

General description

Peptide-N-glycosidase F (PNGase F) belongs to an enzyme family, that are mainly used for the deglycosylation of N-linked glycans.

Application

PNGase Fast may be used to immobilize in order to perform deglycosylation. It may also be used to immobilize onto methacrylate based monolithic support to release the N-linked carbohydrate moieties from glycoproteins.

Biochem/physiol Actions

Peptide-N-glycosidase F (PNGase F) cleaves asparagine-linked high mannose, hybrid and complex oligosaccharides from glycoproteins. It can also deaminate the asparagine to aspartic acid. PNGase Fast enables complete and rapid deglycosylation of antibodies and immunoglobulin fusion proteins, as well as other glycoproteins, to be prepared for downstream chromatography or mass spectrometry analysis. PNGase Fast creates an optimized workflow, reducing processing time without compromising sensitivity or reproducibility.

Storage Class

10 - Combustible liquids

Regulatory Information

常规特殊物品

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Certificates of Analysis (COA)

Lot/Batch Number
SLBV7894

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Articles

Strategies for Deglycosylating N-Linked Glycans

Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.

Rapid Glycan Prep & Analysis by LC-MS Workflow

PNGase Fast denaturing buffer and enzyme provide results similar to a conventional 20-hour protocol, reducing workflow time to about 1 hour.

Deglycosylation Kits

Find deglycosylation kits for your research. Review the different features of deglycosylation kits for glycoproteins, enzymatic proteins, and native proteins.

View All Articles
David A Fischler et al.
Journal of biomolecular techniques : JBT, 30(4), 58-63 (2019-10-11)
There are several methods, both chemical and enzymatic, to release N-linked glycans for structural characterization. One of the most common enzymatic release methods is the use of peptide:N-glycosidase F (PNGase F). A less expensive and quicker alternative has been reported
Kathleen L Plona et al.
JIMD reports, 60(1), 56-66 (2021-07-15)
Due to advances in sequencing technologies, identification of genetic variants is rapid. However, the functional consequences of most genomic variants remain unknown. Consequently, variants of uncertain significance (VUS) that appear in clinical DNA diagnostic reports lack sufficient data for interpretation.

Global Trade Item Number

SKUGTIN
EMS0001-1KT04061824810844