Anti-Mouse IgG (whole molecule) F(ab′)₂ fragment–FITC antibody produced in sheep

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Anti-Mouse IgG (whole molecule) F(ab′)₂ fragment-FITC antibody is specific for ) F(ab′)₂ fragment of mouse IgG1, G2a, G2b, and G3 subclasses. The antibody preparation is solid phase adsorbed with human serum proteins prior to conjugation to minimize cross reactivity. The antibody preparation is conjugated to Fluorescein Isothiocyanate (FITC), Isomer I.

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice
Anti-Mouse IgG (whole molecule) F(ab′)₂ fragment is specific for mouse IgG subclasses G1, G2a, G2b, and G3 as demonstrated by Ouchterlony double diffusion using mouse myeloma proteins. The antibody product can be used to avoid background staining due to the presence of Fc receptors.

Mouse IgG

Pepsin digest of antiserum

Anti-Mouse IgG (whole molecule) F(ab′)₂ fragment-FITC antibody may be used in direct immunofluorescence at a minimum antibody dilution of 1:128. The antibody was used at a working dilution of 1:25 to stain microtubules in epidermal cells of Vigna unguiculata or cowpea It was also used for flow cytometry analysis using COS cells and human peripheral blood mononuclear cells.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)

Binds all mouse Igs.
Useful when trying to avoid background staining due to the presence of Fc receptors.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Adsorbed to reduce background staining with human samples.

Antibody adsorbed with human serum proteins.

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