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F5803

o-Dianisidine dihydrochloride

Name: <I>o</I>-Dianisidine dihydrochloride
Brand: SIGMA

Suitable for use in glucose determination

Synonym(s):

3,3′-Dimethoxybenzidine dihydrochloride, Fast Blue B

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About This Item

Empirical Formula (Hill Notation):

C₁₄H₁₆N₂O₂ · 2HCl

CAS Number:

20325-40-0

Molecular Weight:

317.21

UNSPSC Code:

12171500

NACRES:

NA.47

PubChem Substance ID:

24894894

EC Number:

243-737-5

Beilstein/REAXYS Number:

3917996

MDL number:

MFCD00012488

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Properties

Quality Level

200

form

crystalline

pH range

6.95-7.05

mp

268 °C

solubility

water: 2.5 mg/mL, colorless

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

Cl.Cl.COc1cc(ccc1N)-c2ccc(N)c(OC)c2

InChI

1S/C14H16N2O2.2ClH/c1-17-13-7-9(3-5-11(13)15)10-4-6-12(16)14(8-10)18-2;;/h3-8H,15-16H2,1-2H3;2*1H

InChI key

UXTIAFYTYOEQHV-UHFFFAOYSA-N

Description

General description

A preweighed vial containing 50 mg o-Dianisidine.

Application

o-Dianisidine dihydrochloride is suitable for use in the glucose determination. It is suitable reagent used in the following studies:

As substrate for the determination of serum ceruloplasmin from its oxidase activity.
Determination of total antioxidant response (TAR) against potent free radical reactions by a novel, colorimetric and fully automated method.
As colorimetric indicator for the simultaneous semi-quantitative estimation of glucose, lactate and uric acid on paper-based microfluidic devices (microPAD).
Myeloperoxidase activity assay.

Biochem/physiol Actions

o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.