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F8687

Fetal Bovine Serum

Name: Fetal Bovine Serum
Brand: SIGMA

Australia origin, γ-irradiated, sterile-filtered, suitable for cell culture, suitable for hybridoma

Synonym(s):

Fetal Bovine Serum, FBS, Fetal Bovine Sera, GI FBS, GI FCS, GI Fetal Bovine Sera, GI Fetal Bovine Serum, FCS, sera, serum

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About This Item

UNSPSC Code:

12352207

MDL number:

MFCD00132239

Biological source:

bovine fetus

Origin:

Australia origin

Sterility:

γ-irradiated, sterile-filtered

Shipped in:

dry ice

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Properties

biological source

bovine fetus

Quality Level

500

sterility

γ-irradiated, sterile-filtered

product line

FBS Classic

composition

hemoglobin, ≤0.18 mg/mL

origin

Australia origin

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

impurities

≤5 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

Description

Application

Fetal Bovine Serum has been used in the Roswell Park memorial institute medium-1640 (RPMI-1640) medium to culture myeloma cell lines, RPMI-8226 and U-266. It has also been used as a supplement in Dulbecco′s modified Eagle medium (DMEM) to culture human umbilical vein endothelial cells (HUVECs) and 293 T cells.

Biochem/physiol Actions

Fetal Bovine Serum (FBS) is the commonly used serum to supplement cell culture media due to its high nutritional content. FBS, despite its low protein content, is effective in promoting and maintaining the growth of vertebrate mammalian and insect cells. It can be used as a supplement to culture embryonic stem (ES) cells. FBS can also be used to prepare feeder layers and conditioned media.

Analysis Note

Endotoxin and hemoglobin tested

Other Notes

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

WXBD5226V

WXBD0264V

WXBD0055V

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