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G0762

β-Glucuronidase from Helix pomatia

Type H-3AF, aqueous solution, ≥60,000 units/mL

Synonym(s):

β-D-Glucuronide glucuronosohydrolase

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About This Item

CAS Number:
9001-45-0
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
3.2.1.31 (BRENDA, IUBMB)
MDL number:
MFCD00130629
Specific activity:
≥60,000 units/mL
Biological source:
Helix pomatia
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biological source

Helix pomatia

Quality Level

300

type

Type H-3AF

form

aqueous solution

specific activity

≥60,000 units/mL

impurities

≤1,000 unit/mL Sulfatase

shipped in

wet ice

storage temp.

2-8°C

Application

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Biochem/physiol Actions

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Physical form

Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.

Analysis Note

Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Other Notes

A further processing of Type H-3 to remove agglutinin.
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

低风险生物材料

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Certificates of Analysis (COA)

Lot/Batch Number
SLCJ7821
SLCH9402
SLCC3866
SLBZ7722
SLBW7690

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Protocols

酶促检测罗马蜗牛和牛肝脏来源ß-葡萄糖醛酸酶(EC 3.2.1.31)

酶促检测罗马蜗牛和牛肝脏来源ß-葡萄糖醛酸酶(EC 3.2.1.31)适用于可检测罗马蜗牛和牛肝脏来源ß-葡萄糖醛酸酶的所有产品。应由经过培训的分析服务实验室人员负责按照书面规定执行此程序。

ß-Glucuronidase Enzymatic Assay

Enzymatic assay protocol for β-Glucuronidase from Helix Pomatia and Bovine Liver aids laboratory personnel in following procedures.

Articles

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

W Lechner et al.
Acta endocrinologica, 109(2), 266-268 (1985-06-01)
Unconjugated and total oestriol (E3) have been determined in saliva (S) and plasma (P) by radioimmunoassay using a highly specific antiserum against E3. Total oestrogens (E = E1 + E2 + E3) in urine were analysed by the Kober-Ittrich method.
Tracey B Tucker Zhou et al.
The Journal of biological chemistry, 288(51), 36302-36311 (2013-11-13)
Klotho (KL) is an age-regulating protein named after the Greek goddess who spins the thread of life. Mice deficient in KL are normal throughout development, but rapidly degenerate and display a variety of aging-associated abnormalities that eventually lead to decreased
G Cassinelli et al.
Biochemical pharmacology, 85(10), 1424-1432 (2013-03-08)
The activity of heparanase is responsible for heparan sulfate cleavage, thus resulting in the release of heparan sulfate-bound growth factors. Since heparanase activity is upregulated in several tumor types and is implicated in the malignant behavior, the enzyme is regarded
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Global Trade Item Number

SKUGTIN
G0762-100KU04061833624265