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G1512

β-Glucuronidase from Helix pomatia

Type H-5, lyophilized powder, ≥400,000 units/g solid

Synonym(s):

β-D-Glucuronide glucuronosohydrolase

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About This Item

CAS Number:

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-606-8

MDL number:

EC Number:

3.2.1.31 (BRENDA, IUBMB)

Specific activity:

≥400,000 units/g solid

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Properties

type

Type H-5

Quality Level

form

lyophilized powder

specific activity

≥400,000 units/g solid

secondary activity

≤40,000 units/g solid sulfatase

storage temp.

−20°C

Description

General description

β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Application

β-Glucuronidase from Helix pomatia has been used:

in the enzymatic treatment of urine samples for liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) analysis
in the quercetin extraction procedure from pig or rat tissues for high performance liquid chromatography (HPLC) analysis
in the enzymatic deconjugation step in urine and plasma sample preparation for gas chromatography-mass spectrometry analysis (GC-MS)

β-glucuronidase was used to hydrolyze conjugated glucuronide and sulfate metabolites.

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Biochem/physiol Actions

β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Analysis Note

Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Other Notes

A further purification of G0876 by gel permeation chromatography.

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).

Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

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pictograms

Health hazard

signalword

Danger

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

hcodes

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

wgk

WGK 1

Regulatory Information

低风险生物材料

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Protocols

使用UHPLC/MS（TOF）检测不同酶水解条件处理的尿液中药物和代谢物

为了使用β-葡糖醛酸酶优化水解，必须对每种待分析的葡糖苷酸代谢物进行某些因素的评估，例如孵育时间、温度、水解pH、酶源和酶浓度。

酶促检测罗马蜗牛和牛肝脏来源ß-葡萄糖醛酸酶(EC 3.2.1.31)

酶促检测罗马蜗牛和牛肝脏来源ß-葡萄糖醛酸酶(EC 3.2.1.31)适用于可检测罗马蜗牛和牛肝脏来源ß-葡萄糖醛酸酶的所有产品。应由经过培训的分析服务实验室人员负责按照书面规定执行此程序。

UHPLC/MS for Drug Detection in Urine

Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.

View All Protocols

Articles

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Related Content

Data Sheet - G1512

Tissue distribution of quercetin in rats and pigs

de Boer VCJ, et al.

The Journal of Nutrition, 135(7), 1718-1725 (2005)

GC-MS methods for metabolic profiling of microbial fermentation products of dietary polyphenols in human and in vitro intervention studies

Grun CH, et al.

Journal of Chromatography. B, Biomedical Sciences and Applications, 871(2), 212-219 (2008)

Measurement of intact sulfate and glucuronide phytoestrogen conjugates in human urine using isotope dilution liquid chromatography-tandem mass spectrometry with [13C3] isoflavone internal standards

Clarke DB, et al.

Analytical Biochemistry, 309(1), 158-172 (2002)

View All Related Papers

Global Trade Item Number

SKU	GTIN
G1512-100KU	04061833628188
G1512-500KU	04061835512553