G1924
L-Glutamate Oxidase from Streptomyces sp.
recombinant, expressed in E. coli
Synonym(s):
L-Glutamate:oxygen oxidoreductase (deaminating)
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About This Item
Specific activity:
≥5 units/mg protein
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
Quality Level
form
solid
specific activity
≥5 units/mg protein
mol wt
120 kDa
storage temp.
−20°C
General description
L-Glutamate Oxidase (LGOX) from Streptomyces sp is secreted extracellularly in the wheat bran pre cultivation medium. It corresponds to a molecular weight of 120 kDa with monomer subunit of 61 kDa. The structure of LGOX comprises of hexamer and has flavin adenine dinucleotide prosthetic group.
This product has a 6xHistidine tag with a thrombin cleavage site.
Application
L-Glutamate Oxidase from Streptomyces sp may be used:
- as a reference enzyme in hydrogelpoly(carbamoylsulphonate) based immobilization for biosensor development
- as a component of pencil graphite electrode for biosensing L-glutamate
Biochem/physiol Actions
L-Glutamate Oxidase (LGOX) catalyzes the oxidative deamination of L-glutamate to α-ketoglutarate, ammonia and hydrogen peroxide. LGOX is industrially produced and is exploited majorly as a biosensor for L-glutamate detection and in the L-glutamate kits.
Other Notes
One unit will form 1.0 micromole of alpha-ketoglutaric acid from L-glutamic acid per minute at pH 7.4 at 30 deg C.
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
常规特殊物品
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Comparative study of hydrogel-immobilized L-glutamate oxidases for a novel thick-film biosensor and its application in food samples
Kwong AWK, et al.
Biotechnology Letters, 22(4), 267-272 (2000)
L-Glutamate biosensor based on l-glutamate oxidase immobilized onto ZnO nanorods/polypyrrole modified pencil graphite electrode
Batra B, et al.
Biochemical Engineering Journal, 105, 428-436 (2016)
Isolation, purification, and characterization of L-glutamate oxidase from Streptomyces sp. 18G
Wachiratianchai S, et al.
Electronic journal of Biotechnology, 7(3), 09-10 (2004)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| G1924-1UN | 04061826703298 |
| G1924-5UN | 04061833628515 |