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G2751

Glutamic-Oxalacetic Transaminase from porcine heart

Name: Glutamic-Oxalacetic Transaminase from porcine heart
Brand: SIGMA

Type I, ammonium sulfate suspension, 200-500 units/mg protein

Synonym(s):

L-Aspartate:2-oxoglutarate aminotransferase, Aspartate Aminotransferase, GOT

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About This Item

CAS Number:

9000-97-9

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-571-9

MDL number:

MFCD00131191

EC Number:

2.6.1.1 (BRENDA, IUBMB)

Specific activity:

200-500 units/mg protein

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Properties

type

Type I

Quality Level

200

form

ammonium sulfate suspension

specific activity

200-500 units/mg protein

foreign activity

glutamic-pyruvic transaminase ≤0.03%, lactic dehydrogenase ≤0.01%, malic dehydrogenase ≤0.01%

shipped in

wet ice

storage temp.

2-8°C

Description

General description

Research area: Cellsignaling. Glutamic-Oxalacetic Transaminase is found in all tissues and is predominant in the liver and skeletal muscle. It is a prototype of fold-type I pyridoxal 5′-phosphate (PLP)-enzymes. Glutamic-oxalacetic transaminase is a homodimer containing large and small domains in each subunit.

Application

Glutamic-OxalaceticTransaminase from porcine heart is suitable for the preparation ofphenylalanine amino acid. The immobilized form of this enzyme maybe used incomparison studies between soluble and immobilized forms in terms of reactionefficiency and stability.

Glutamic-Oxalacetic Transaminase from porcine heart has been used:

in an enzyme-coupled assay for evaluating L-asparaginase enzyme activity
in microtiter plate format enzyme-based assay to estimate malic acid content in berry samples
as a supplement in homogenization buffers for the isolation of heart mitochondrial membranes in the presence of oxaloacetate (OAA)-depleting system

Biochem/physiol Actions

Glutamic-oxalacetic transaminase or aspartate aminotransferase (AAT) catalyzes the interconversion of L-aspartate and α-ketoglutarate with oxalacetate and L-glutamate. This reversible transaminase reaction is dependent on pyridoxal 5′-phosphate (PLP). Glutamic-oxalacetic transaminase maintains the nitrogen currency for metabolism by generating L-glutamate. Elevated serum levels of glutamic-oxalacetic transaminase are indicated in myocardial infarction, liver diseases, and some renal diseases.

Physical form

Suspension in 3.0 M (NH₄)₂SO₄ containing 0.05 M maleate and 2.5 mM α-ketoglutarate, pH 6.0

Analysis Note

Protein determined by biuret.

Other Notes

One unit will convert 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.5 at 37 °C, in the presence of L-aspartic acid. One unit is equivalent to ~2,000O.D. (Karmen) units at 25 °C.