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G5885

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

Name: Glucose-6-phosphate Dehydrogenase from <I>Leuconostoc mesenteroides</I>
Brand: SIGMA

lyophilized powder, >= 550 units/mg protein (biuret)

Synonym(s):

G-6-P-DH

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About This Item

CAS Number:

9001-40-5

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-602-6

MDL number:

MFCD00081656

EC Number:

1.1.1.49 (BRENDA, IUBMB)

Specific activity:

>= 550 units/mg protein (biuret)

Biological source:

bacterial (Leuconostoc mesenteroides)

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Properties

biological source

bacterial (Leuconostoc mesenteroides)

Quality Level

300

type

Type XXIV

form

lyophilized powder

specific activity

>= 550 units/mg protein (biuret)

mol wt

128 kDa

composition

Protein, 15-40% biuret

application(s)

agriculture

foreign activity

6-Phosphogluconic dehydrogenase, hexokinase, NADH oxidase and NADPH oxidase ≤0.005%, PGI ≤0.01%

storage temp.

2-8°C

Description

General description

Glucose-6-Phophate Dehydrogenase (G-6-PDH) comprises His-Asp catalytic dyad and exists as a homodimer. Structurally, G-6-PDH encompasses a Rossmann dinucleotide binding fold in the coenzyme binding domain region. It also harbors a large β + α domain and has a unique aspartate residue at position 374.

Application

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides has been used along with hexokinase in the determination of glucose from mice liver samples.

Biochem/physiol Actions

Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Glucose-6-Phophate Dehydrogenase (G-6-PDH) can utilize either nicotinamide adenine dinucleotide phosphate (NADP⁺) or NAD⁺ as coenzyme making it crucial for bacterial metabolism.

Physical form

Lyophilized powder containing Ficoll and Tris buffer salts

Other Notes

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.

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pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Protocols

6-磷酸葡萄糖脱氢酶（EC 1.1.1.49）的酶学测定

在测定6-磷酸葡萄糖脱氢酶活性时，使用β-烟酰胺腺嘌呤二核苷酸磷酸在340 nm处通过分光光度法进行测定。

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.

Global Trade Item Number

SKU	GTIN
G5885-2KU	04061833638620
G5885-10KU	04061832729893
G5885-1KU	04061833638606
G5885-200UN	04061833638613