β-Glucuronidase from Helix pomatia

Name: β-Glucuronidase from <I>Helix pomatia</I>
Brand: SIGMA

Type HP-2S, aqueous solution, ≥90,000 units/mL

Synonym(s):

β-D-Glucuronide glucuronosohydrolase

Select a Size

Change View

About This Item

CAS Number:

9001-45-0

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

3.2.1.31 (BRENDA, IUBMB)

MDL number:

MFCD00130629

Specific activity:

≥90,000 units/mL

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

sterility

sterile-filtered

Quality Level

200

type

Type HP-2S

form

aqueous solution

specific activity

≥90,000 units/mL

secondary activity

≤7,500 units/mL sulfatase

storage temp.

2-8°C

Description

General description

β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Application

β-Glucuronidase from Helix pomatia has been used:

in the treatment of heparinized plasma sample for the hydrolysis of glucuronic acid-conjugated metabolites
in the pre-treatment of plasma sample for the determination of 1′-hydroxymidazolam (1′-OHMDZ)
for the treatment of bile samples from rainbow trouts for the hydrolysis of glucuronate and sulfate metabolites
in treating the cells from the sporulation media for the complete digestion of the cell walls of asci

β-glucuronidase was used in enzymic hydrolysis of tissue homogenates for liquid chromatography-electrospray ion trap mass spectrometry (LC/MSⁿ) analysis, to study the structures of degradation products of baicalin. It is used as a reporter gene in GUS assays to monitor gene expression.

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Biochem/physiol Actions

β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis