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G7877

Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)

Name: Glucose-6-phosphate Dehydrogenase from baker′s yeast (<I>S. cerevisiae</I>)
Brand: SIGMA

Type VII, ammonium sulfate suspension, ≥200 units/mg protein

Synonym(s):

glucose-6-phosphate dehydrogenase (NADP+), G-6-P-DH, Zwischenferment

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About This Item

CAS Number:

9001-40-5

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-602-6

MDL number:

MFCD00081656

EC Number:

1.1.1.49 (BRENDA, IUBMB)

Specific activity:

≥200 units/mg protein

Biological source:

bakers yeast

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Properties

biological source

bakers yeast

Quality Level

200

type

Type VII

form

ammonium sulfate suspension

specific activity

≥200 units/mg protein

mol wt

128 kDa

technique(s)

cell based assay: suitable

solubility

water: soluble

application(s)

diagnostic assay manufacturing

storage temp.

2-8°C

Gene Information

Saccharomyces cerevisiae ... ZWF1(855480)

Description

General description

Glucose 6-phosphate dehydrogenase (G-6-P-DH) is a key regulatory enzyme in the first step of the pentose phosphate pathway. G-6-P-DH is a glycoprotein with a molecular mass of 128 kDa (gel filtration).

Application

Glucose-6-phosphate dehydrogenase has been used in a study to investigate the effect of dehydroepiandrosterone on cell growth and mitochondrial function in TM-3 cells. Glucose-6-phosphate dehydrogenase has also been used in a study to investigate the incidence of apoptosis and transcript abundance in bovine follicular cells. Glucose-6-phosphate dehydrogenase is used to test ketose reductase activity in developing maize endosperm.

Biochem/physiol Actions

Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Physical form

Crystalline suspension in 3.2 M (NH₄)₂SO₄ solution, pH 7.0

Other Notes

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NADP at pH 7.4 at 25 °C.

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pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

常规特殊物品

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Protocols

6-磷酸葡萄糖脱氢酶（EC 1.1.1.49）的酶学测定

在测定6-磷酸葡萄糖脱氢酶活性时，使用β-烟酰胺腺嘌呤二核苷酸磷酸在340 nm处通过分光光度法进行测定。

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.

Articles

如何使用溶于硫酸铵悬液的酶

以硫酸铵悬浮液形式提供的酶的使用指南

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Global Trade Item Number

SKU	GTIN
G7877-1KU	04061833643310
G7877-5KU	04061833643440
G7877-150UN	04061832730196
G7877-250UN	04061833643334
G7877-2KU	04061833643341
G7877-500UN	04061833643358