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G8168

G418 disulfate salt

Name: G418 disulfate salt
Brand: SIGMA

solution, non-animal origin, suitable for cell culture, BioReagent

Synonym(s):

Antibiotic G418

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About This Item

Empirical Formula (Hill Notation):

C₂₀H₄₀N₄O₁₀ · 2H₂SO₄

CAS Number:

108321-42-2

Molecular Weight:

692.71

MDL number:

MFCD00058314

UNSPSC Code:

12352207

PubChem Substance ID:

24895333

NACRES:

NA.76

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Properties

Product Name

G 418 disulfate salt solution, 50 mg/mL in H₂O, 0.1 μm filtered, BioReagent, suitable for cell culture

biological source

Micromonospora echinospora

sterility

0.1 μm filtered

product line

BioReagent

form

solution

potency

50-60 mg per mL

mol wt

692.7

packaging

pkg of 10 and 100 mL

concentration

50 mg/mL in H₂O

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

colorless

solubility

H₂O: 50 mg/mL, clear

antibiotic activity spectrum

parasites

mode of action

protein synthesis | interferes

storage temp.

2-8°C

SMILES string

OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O

InChI

1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1

InChI key

UHEPSJJJMTWUCP-NKCAIAFTSA-N

Description

General description

Chemical structure: aminoglycoside

Application

G418 disulfate is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL.

Biochem/physiol Actions

Mode of Action: G418 blocks polypeptide synthesis and protein elongation by inhibiting synthesis at the 70S and 80S ribosomes.

Antimicrobial Spectrum: G418 selects for cells stably transfected with an iNOS promoter construct and neomycin resistance gene and shows activity against protozoa and helminthes.