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G8295

β-Glucuronidase from Escherichia coli

Name: β-Glucuronidase from <I>Escherichia coli</I>
Brand: SIGMA

≥10,000,000 units/g protein (30 min assay), recombinant, expressed in E. coli overproducing strain, lyophilized powder

Synonym(s):

β-D-Glucuronide glucuronosohydrolase

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About This Item

CAS Number:

9001-45-0

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-606-8

MDL number:

MFCD00199013

EC Number:

3.2.1.31 (BRENDA, IUBMB)

Specific activity:

≥10,000,000 units/g protein (30 min assay)

Recombinant:

expressed in E. coli overproducing strain

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Properties

recombinant

expressed in E. coli overproducing strain

Quality Level

300

form

lyophilized powder

specific activity

≥10,000,000 units/g protein (30 min assay)

mol wt

~290 kDa

composition

protein, 40-60% biuret

storage temp.

−20°C

Description

Application

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Effective in the hydrolysis of steroid glucuronides.

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Analysis Note

Contains buffer salts and stabilizer.

Other Notes

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).

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pictograms

GHS08

signalword

Danger

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

wgk

WGK 1

Regulatory Information

常规特殊物品

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Protocols

使用UHPLC/MS（TOF）检测不同酶水解条件处理的尿液中药物和代谢物

为了使用β-葡糖醛酸酶优化水解，必须对每种待分析的葡糖苷酸代谢物进行某些因素的评估，例如孵育时间、温度、水解pH、酶源和酶浓度。

UHPLC/MS for Drug Detection in Urine

Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.

Enzymatic Assay of β-Glucuronidase (EC 3.2.1.31) from E. coli

Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.

Articles

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Probiotics and Human Health

Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.

Global Trade Item Number

SKU	GTIN
G8295-25KU	04061833218792
G8295-2MU	04061833218808
G8295-500KU	04061833218815