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G8420

β-Glucuronidase from Escherichia coli

≥20,000 units/mg protein, recombinant, expressed in E. coli overproducing strain, lyophilized powder

Synonym(s):

β-D-Glucuronide glucuronosohydrolase

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About This Item

CAS Number:
9001-45-0
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-606-8
MDL number:
MFCD00199013
EC Number:
3.2.1.31 (BRENDA, IUBMB)
Specific activity:
≥20,000 units/mg protein
Recombinant:
expressed in E. coli overproducing strain
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recombinant

expressed in E. coli overproducing strain

Quality Level

300

form

lyophilized powder

specific activity

≥20,000 units/mg protein

mol wt

290 kDa

composition

, ~25%

storage temp.

−20°C

Application

Effective in the hydrolysis of steroid glucuronides.
New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Analysis Note

Contains buffer salts and stabilizer.

Other Notes

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).

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pictograms

Health hazard
GHS08

signalword

Danger

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

hcodes

H334

Hazard Classifications

Resp. Sens. 1

wgk

WGK 1

Regulatory Information

常规特殊物品

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Certificates of Analysis (COA)

Lot/Batch Number
50180211
TEST
SLBQ0687V
078K7700V
078K7700

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Protocols

Enzymatic Assay of β-Glucuronidase (EC 3.2.1.31) from E. coli

Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.

Articles

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Probiotics and Human Health

Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.

Related Content

Enzymes
Hans Ulrich Bergmeyer
Methods of Analysis, 2, 460-461 (1974)
V Graef et al.
Clinical chemistry, 23(3), 532-535 (1977-03-01)
We determined the enzymic activity of beta-glucuronidase preparations from bovine liver, Helix pomatia, and Escherischia coli with steroid glucuronides and nonsteroid glucuronides as substrates. We also studied the effect of Na2SO4 on the enzymic hydrolysis of several substrates with the
Xiaoyan Liu et al.
Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences, 42(1), 67-74 (2013-03-19)
To construct a lentiviral RNA interference system targeting heparanase (HPSE) based on miR30 and to test its silencing effect. Three heparanase-shRNA structures were designed based miR30. The targeting fragments were obtained by PCR, then inserted into the vector LV PP-GFP
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Global Trade Item Number

SKUGTIN
G8420-25KU04061833218839
G8420-100KU04061833218822