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GE17-0110-01

Sepharose 6B

Cytiva 17-0110-01, pack of 1 L

Synonym(s):

Agarose, Beaded

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About This Item

CAS Number:
UNSPSC Code:
41106500
NACRES:
NA.56
MDL number:
Technical Service
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feature

autoclavable Autoclavable, 20 min at 120°C in pH 7

packaging

pack of 1 L

manufacturer/tradename

Cytiva 17-0110-01

matrix

6% agarose

particle size

45-165 μm

cleaning in place

4-9

working range

4-9

SMILES string

OC[C@H]1O[C@@H](O[C@@H]2[C@@H]3CO[C@H]2[C@H](O)[C@@H](O3)O[C@H]4[C@@H](O)[C@@H](CO)O[C@@H](O[C@@H]5[C@@H]6CO[C@H]5[C@H](O)[C@H](O)O6)[C@@H]4O)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

General description

Sepharose 6B is a well-proven agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.

Sepharose is a bead-formed agarose-based gel filtration matrix. Sepharose is available with 3 different agarose contents; 2, 4, and 6%, designated Sepharose 2B, Sepharose 4B and Sepharose 6B respectively, Both Sepharose and Sepharose CL have broad fractionation ranges which makes them suitable for characterizing or cleaning-up samples containing components of diverse molecular weight.

Sepharose CL gels are cross-linked derivatives of Sepharose 2B, Sepharose 4B and Sepharose 6B. The cross-linked form of Sepharose is chemically and physically more resistant than Sepharose itself, offering the same selectivity with better flow characteristics. Cross-linked Sepharose gels are resistant to organic solvents and are thus the choice for separations in organic solvents.

Features and Benefits

  • 6% Agarose gel filtration media.
  • Proven base matrix for coupling affinity ligands

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

Beaded agarose for fractionating molecules of high molecular weight. Cross-linked beaded agarose is more resistant to denaturing conditions, and thus offers more versatility in the choice of sample buffer and eluent. The approximate % agarose concentration is indicated by the product name (Ultrogel A%).

Legal Information

Sepharose is a trademark of Cytiva


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Storage Class

3 - Flammable liquids

Regulatory Information

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The agarose migration inhibition technique for in vitro demonstration of cell-mediated immunity in man. A review.
J E Clausen
Danish medical bulletin, 22(5), 181-194 (1975-07-01)
Jana Wolf et al.
The EMBO journal, 33(14), 1514-1526 (2014-05-30)
The conserved eukaryotic Pan2-Pan3 deadenylation complex shortens cytoplasmic mRNA 3' polyA tails to regulate mRNA stability. Although the exonuclease activity resides in Pan2, efficient deadenylation requires Pan3. The mechanistic role of Pan3 is unclear. Here, we show that Pan3 binds
Sudhir Chandna et al.
International journal of radiation biology, 90(5), 401-406 (2014-02-18)
Primary fibroblasts are not suitable for in vitro macrocolony assay due to their inability to form distinct colonies. Here we present a modification of agarose overlay that yielded extensive improvement in their colony formation and assessment of radiosensitivity. Macrocolony formation



Global Trade Item Number

SKUGTIN
GE17-0110-0104061837620003