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GERPN2209

ECL Western Blotting Detection Reagents

Cytiva RPN2209

Synonym(s):

ECL detection reagents, Western blot reagents

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About This Item

NACRES:
NA.32
UNSPSC Code:
41105332
Technical Service
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feature

wetted part: no

packaging

pkg of 1 kit

manufacturer/tradename

Cytiva RPN2209

color

Detection reagent 1: Colorless Detection reagent 2: Colorless

storage temp.

2-8°C

General description

Amersham ECL Western Blotting Detection Reagents were the world′s first commercial chemiluminescent reagents for Western blotting and are still the most widely used. They are at least 10-fold more sensitive than colorimetric assays and produce excellent and reliable results every time.

These reagents use enhanced luminol-based detection which is suitable for all routine western blotting. ECL Western Blotting Detection Reagents in combination with Hybond® ECL membranes form a complete solution for Western blot applications.

Contents include the following:
GERPN2109, 62.5 mL each of ECL Detection Reagents 1 and 2 (1000 cm2)
GERPN2209, 125 mL each (2000 cm2)
GERPN2106, 250 mL each (4000 cm2)
GERPN2134, 375 mL each (6000 cm2)
Emission duration max. 2 h

Application

Western Blotting

Features and Benefits

  • High sensitivity, ~10 pg in model system.
  • Proven performance, the most frequently cited ECL Western Blotting Detection Reagents in publications
  • Detectable on Hyperfilm ECL or with CCD imager
  • Recommended mebrane Hybond ECL

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

Sufficient for detection of 2000 cm2 of membrane:
125 mL Detection reagent 1(color : colorless)
125 mL Detection reagent 2 (color: colorless)

Legal Information

Amersham is a trademark of Cytiva
ECL is a trademark of Cytiva
Hybond is a registered trademark of Cytiva


Storage Class

12 - Non Combustible Liquids

Regulatory Information

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Certificates of Analysis (COA)

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Protocols

This page describes chemiluminescence detection of GST-tagged proteins with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select from Cytiva.

This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using Cytiva products.

Articles

描述被分子生物学家用于检测转印膜上蛋白质和核酸的方法的背景和实验方案。

This page describes troubleshooting strategies in the detection of GST-tagged proteins.

Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.


Qinmiao Huang et al.
Molecular medicine reports, 16(5), 7086-7091 (2017-09-14)
Lung cancer is reported to be a major public health issue worldwide and the overall prognosis of patients remains poor. The expression levels of Livin and Survivin, of the inhibitors of apoptosis (IAP) family, are associated with prognostic significance in
Hua Zhang et al.
International immunopharmacology, 69, 88-94 (2019-01-28)
Inflammation is fundamental in osteoarthritis (OA) pathogenesis. Semaphorin 4A (Sema4A) has been implicated in immune-associated diseases, however, its role in OA remains unclear. In this study, we show that Sema4A is upregulated in knee OA articular cartilage as well as



Global Trade Item Number

SKUGTIN
GERPN220904061834084969