Key Documents

View All Documentation

H1136

Hyaluronidase from Streptomyces hyalurolyticus

Name: Hyaluronidase from <I>Streptomyces hyalurolyticus</I>
Brand: SIGMA

Synonym(s):

Hyaluronate Lyase from Streptomyces hyalurolyticus

Select a Size

Change View

About This Item

CAS Number:

37259-53-3

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

253-430-8

MDL number:

MFCD00131347

EC Number:

4.2.2.1 (BRENDA, IUBMB)

Biological source:

Streptomyces sp. (S. hyalurolyticus)

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

Streptomyces sp. (S. hyalurolyticus)

Quality Level

200

conjugate

conjugate (Glucosaminoglycan)

form

film (Clear)

packaging

ampule of 300 × units

storage temp.

−20°C

Description

General description

Hyaluronidase is widely present in nature. In humans, it is present in a number of organs and body fluids. Hyaluronidase is found in the secretions of virus, bacteriophage, fungi and bacteria. It is also present in nematode and leeches secretions. Snakes and scorpions produce hyaluronidase in their venom. Hyaluronidase is also secreted by bees, spiders, wasps, hornets, caterpillars, fishes and lizards.

Application

Hyaluronidase from Streptomyces hyalurolyticus has been used:

in the preparation of DMEM/F-12 (Dulbecco′s modified eagle medium/nutrient mixture F-12) media for the isolation and purification of single cells from dissociated tumour tissue
as a component of digestion solution for the derivation of germline stem cells from testicular tissue

Biochem/physiol Actions

Hyaluronidase is an endoglycosidase and performs its function along with exoglycosidases, like β-glucuronidase and β-N-acetyl glucosaminidase. Hyaluronidase regulates the hyaluronic acid turnover.

Hyaluronate lyase cleaves hyaluronic acid at the β-D-GlcNAc-(1→4)-β-D-GlcA bond, yielding 3-(4-deoxy-β-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine tetra- and hexasaccharides. Unlike other hyaluronidases, this enzyme is specific for hyaluronic acid and is inactive with chondroitin and chondroitin sulfate.

Other Notes

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

wgk

WGK 3

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

常规特殊物品

This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Protocols

透明质酸酶的酶法测定 (3.2.1.35)

在测定透明质酸酶活性时，使用了一种在600 nm处的比浊测定法进行测定。在pH 5.35、37 °C下，一单位的透明质酸酶活性可每分钟引起0.330的吸光值改变。

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

Articles

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

Glycosaminoglycans and Proteoglycans

Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.

What is Hyaluronan?

Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.

Global Trade Item Number

SKU	GTIN
H1136-1AMP	04061833663936
H1136-5X1AMP	04061833663950