Monoclonal Anti-HLA Class I Antigen antibody produced in mouse

Class I proteins are highly polymorphic, in which HLA-B seems to be the most polymorphic gene in the human genome. HLA-A, -B, -C are classical class I proteins and are heterodimers. HLA-A, -B, -C consists of a polymorphic transmembrane α heavy chain and an invariant water-soluble light chain. HLA-B is located on human chromosome 6p21.

The previously assigned protein identifier P01891 has been merged into P04439. Full details can be found on the UniProt database.

human tonsil leukocyte membrane preparation.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

HLA class I molecules like HLA-A, -B and −C help in the presentation of endogenous antigen to CD8+ (cluster of differentiation 8) T cells.

Recognizes the human HLA Class I antigen expressed on most human nucleated cells. The antibody reacts with a monomorphic epitope on the 44-45 kDa α-chain polypeptide of human class I HLA molecules (HLA-A,B,C). The antibody may be used in immunoprecipitation, complement mediated cytotoxicity, flow cytometry and immunohistochemical staining of acetone-fixed, frozen sections and cell smears. It cross-reacts with cells from African and Asian apes and old world monkeys. No reactivity is seen with cells from most new world monkeys or from non-primates. The epitope recognized by this antibody is sensitive to routine formalin fixation and paraffin embedding.
4th Workshop: code no. P1
5th Workshop: code nos. BP166, BP288, BP407, B 005

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

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