H2519
Heparinase I from Flavobacterium heparinum
Lyophilized powder stabilized with approx. 25% bovine serum albumin, ≥200 units/mg protein (enzyme + BSA)
Synonym(s):
Heparin lyase I, Heparinase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥200 units/mg protein (enzyme + BSA)
conjugate
conjugate (Glucosaminoglycan)
Quality Level
form
lyophilized solid
specific activity
≥200 units/mg protein (enzyme + BSA)
mol wt
42.8 kDa
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
Application
Heparinase I from Flavobacterium heparinum has been used:
- in the digestion of plasma samples
- to study the effect of medium, washing and sonication on the infectivity of Chlamydia pneumonia
- in enzymatic removal of heparan sulfate from the surface of human rhabdomyosarcoma cells
Biochem/physiol Actions
Heparinase I from Flavobacterium heparinum acts by cleaving the α-glycoside linkage at AT III binding site in heparin. This results in small oligosaccharide fragments . Heparinase I neutralizes the protein′s action of anticoagulation. Heparinase I possesses a half-life of 15-18 minutes.
Other Notes
One unit (Sigma unit) will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
低风险生物材料
常规特殊物品
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Articles
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.
Cardiopulmonary Bypass: Principles and Practice, 509-509 (2008)
Gabriel E Bertolesi et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 240(12), 2657-2672 (2011-11-11)
In Xenopus laevis embryos, heparanase, the enzyme that degrades heparan sulfate, is synthesized as a preproheparanase (XHpaL) and processed to become enzymatically active (XHpa active). A short nonenzymatic heparanase splice variant (XHpaS) is also expressed. Using immunohistochemistry, Western blot, and
Udayanath Aich et al.
Analytical chemistry, 83(20), 7815-7822 (2011-08-26)
Heparin and the low molecular weight heparins are extensively used as medicinal products to prevent and treat the formation of venous and arterial thrombi. In early 2008, administration of some heparin lots was associated with the advent of severe adverse
Global Trade Item Number
| SKU | GTIN |
|---|---|
| H2519-50UN | 04061833792308 |
| H2519-100UN | 04061833792285 |
| H2519-250UN | 04061833792292 |