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H3757

Hyaluronidase from bovine testes

Type VIII, lyophilized powder, 300-1,000 U/mg

Synonym(s):

Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase

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About This Item

CAS Number:
37326-33-3
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-464-3
MDL number:
MFCD00131351
EC Number:
3.2.1.35 (BRENDA, IUBMB)
Specific activity:
300-1,000 U/mg
Biological source:
bovine testis
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biological source

bovine testis

Quality Level

200

conjugate

conjugate (Glucosaminoglycan)

type

Type VIII

form

lyophilized powder

specific activity

300-1,000 U/mg

mol wt

~55 kDa (four subunits of 14 kDa each)

UniProt accession no.

Q5E985, Q8SQG8

relevant disease(s)

cancer

storage temp.

−20°C

Gene Information

cow ... HYAL1(515397), HYAL2(281838)

Biochem/physiol Actions

These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.
Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression.

Preparation Note

Prepared from aseptic filled solution of Type I-S.

Other Notes

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

低风险生物材料

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Certificates of Analysis (COA)

Lot/Batch Number
SLCK7710
SLCG7032
SLBZ6642
SLBX1082
SLBT8168

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Protocols

透明质酸酶的酶法测定 (3.2.1.35)

在测定透明质酸酶活性时，使用了一种在600 nm处的比浊测定法进行测定。在pH 5.35、37 °C下，一单位的透明质酸酶活性可每分钟引起0.330的吸光值改变。

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

Articles

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

Glycosaminoglycans and Proteoglycans

Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.

What is Hyaluronan?

Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.

Kohtaro Miyazawa et al.
Histochemistry and cell biology, 133(1), 125-134 (2009-10-16)
Membranous epithelial cells (M cells) of the follicle-associated epithelium in Peyer's patches have a high capacity for transcytosis of several viruses and microorganisms. Here, we report that we have successfully established a bovine intestinal epithelial cell line (BIE cells) and
C Rollo et al.
Reproduction (Cambridge, England), 154(4), 375-385 (2017-09-08)
Acetylation of histone proteins is a major determinant of chromatin structure and function. Fertilisation triggers a round of chromatin remodelling that prepares the genome for the first round of transcription from the new embryonic genome. In this study we confirm
Carmen A Vlahu et al.
Journal of the American Society of Nephrology : JASN, 23(11), 1900-1908 (2012-10-23)
Damage to the endothelial glycocalyx, which helps maintain vascular homeostasis, heightens the sensitivity of the vasculature to atherogenic stimuli. Patients with renal failure have endothelial dysfunction and increased risk for cardiovascular morbidity and mortality, but the state of the endothelial
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Global Trade Item Number

SKUGTIN
H3757-10X100MG04061826649787
H3757-100MG04061835545483