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H6147

HEPES

Name: HEPES
Brand: SIGMA

BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)

Synonym(s):

4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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About This Item

Empirical Formula (Hill Notation):

C₈H₁₈N₂O₄S

CAS Number:

7365-45-9

Molecular Weight:

238.30

UNSPSC Code:

12161704

NACRES:

NA.75

PubChem Substance ID:

24895731

EC Number:

230-907-9

Beilstein/REAXYS Number:

883043

MDL number:

MFCD00006158

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Properties

product line

BioXtra

Quality Level

400

assay

≥99.5% (titration)

form

powder

technique(s)

cell culture | embryo: suitable

pH

5.0-6.5 (25 °C, 238 g/L)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

water: 500 mg/mL, clear, colorless

absorption

≤0.05 at 290 in H₂O at 33%

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg. It is typically used in cell culture at concentration between 5mM to 30mM.

Useful pH range: 6.8 - 8.2

Application

HEPES has been used:

As a constituent of modified Eagle′s minimum essential medium for in vitro culturing of spermatogenic cells and as a constituent of the cryoprotectant used to preserve these germ cells
As a component of modified Ham′s F-10 for the incubation of doe zygotes to carry out Haploid male hemizygous recipient production
in the preparation of 90% Percoll solution used in Percoll- gradient method for sperm collection and preparation

Other Notes

Easily compare specifications for HEPES products with the HEPES specification table.

This product has been qualified for use in cell and mouse embryo culture.

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Embryo Culture Media

Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture

Global Trade Item Number

SKU	GTIN
H6147-100G	04061833800379
H6147-25G	04061833800393
H6147-500G	04061833800416