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Merck
CN

H9269

Hanks′ Balanced Salt solution

With sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

HBSS

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About This Item

NACRES:
NA.75
UNSPSC Code:
12352207
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Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: 0.35 g/L
phenol red: 0.011 g/L
glucose: 1.0 g/L (Dextro)

shipped in

ambient

storage temp.

room temp

General description

Formulation: click here.

Application

Hanks′ balanced salt solution has been used:
  • in starvation experiments for mammalian cells
  • to collect and maintain brain pieces for primary glial cell culture
  • to wash femoral tissue and bone marrow (BM) to culture mesenchymal stem cells

Biochem/physiol Actions

Balanced salt solution (BSS) in cell culture is multifaceted and has various functions. It serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance. BSS provides cells with water and certain bulk inorganic ions essential for normal cell metabolism. It combines with carbohydrates, such as glucose to act as the principal energy source for cell metabolism. BSS also provides a buffering system to maintain the medium within the physiological pH range (7.2-7.6).


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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Protocols

该96孔3D球体BME细胞侵袭检测试剂盒利用符合3D培养要求的96孔球状体形成平板以及专门的球状体形成ECM来促进细胞的聚集和球状体形成。

3D Spheroid BME Cell Invasion Assay utilizes specialized plates and ECM for cell aggregation and spheroid formation in vitro.


Sonya Nassari et al.
Scientific reports, 7(1), 17279-17279 (2017-12-10)
Connective tissues (CT) support and connect organs together. Understanding the formation of CT is important, as CT deregulation leads to fibrosis. The identification of CT specific markers has contributed to a better understanding of CT function during development. In developing
Harald Kerkkamp et al.
Toxicon : official journal of the International Society on Toxinology, 150, 39-49 (2018-05-16)
Currently, biological and organic substances are screened in order to find a new generation of therapeutics active against cancer. Previous research has identified promising candidate peptides in snake venom. In this study, venoms from different snake species (Naja annulifera, Naja
Ruyi Zhang et al.
Liver international : official journal of the International Association for the Study of the Liver, 41(1), 206-219 (2020-10-22)
Mature transfer RNAs (tRNA) charged with amino acids decode mRNA to synthesize proteins. Dysregulation of translational machineries has a fundamental impact on cancer biology. This study aims to map the tRNAome landscape in liver cancer patients and to explore potential



Global Trade Item Number

SKUGTIN
H9269-500ML04061833812297
H9269-6X500ML04061833812389
H9269-100ML04061833812242
H9269-6X1L04061833812334
H9269-1L04061833812273