I3390
IMDM
With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
Iscove′s MDM, Iscoves MDM, Iscove’s Modified Dulbecco’s Medium, IMDM
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About This Item
Product Name
Iscove′s Modified Dulbecco′s Medium, liquid, sterile-filtered, With sodium bicarbonate, without L-glutamine, suitable for cell culture, suitable for hybridoma
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | hybridoma: suitable, cell culture | mammalian: suitable
impurities
endotoxin, tested
components
phenol red: yes
L-glutamine: no
glucose: yes
shipped in
ambient
storage temp.
2-8°C
Application
Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove′s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.
Preparation Note
Supplement with 0.584 g/L L-glutamine
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
N N Iscove et al.
The Journal of experimental medicine, 147(3), 923-933 (1978-03-01)
Albumin, transferrin, and lipids can replace serum entirely for support of LPS-stimulated murine B lymphocytes in culture. In the presence of these compounds, growth and maturation to IgM and IgG secretion, induced by lipopolysaccharide (LPS), occurs at the same or
Kirsten Gillingwater
Parasitology, 145(7), 953-960 (2017-11-24)
Trypanosoma equiperdum is a protozoan parasite responsible for causing Dourine, a debilitating neglected veterinary disease, found worldwide affecting equids. It is the only pathogenic trypanosome species that does not require an invertebrate vector for transmission, thus being passed from animal
S S Ozturk et al.
Journal of biotechnology, 18(1-2), 13-28 (1991-04-01)
A murine hybridoma cell line (167.4G5.3) was cultured in batch mode using IMDM containing different serum concentrations and bovine serum albumin (BSA). Cell growth and death, metabolism and antibody production were studied in these cultures. The cells were more susceptible
Global Trade Item Number
| SKU | GTIN |
|---|---|
| I3390-10L | 04061837340543 |
| I3390-20L | 04061837340550 |
| I3390-24X500ML | 04061833856062 |
| I3390-6X500ML | 04061833856093 |
| I3390-1L | 04061833856055 |
| I3390-500ML | 04061833856079 |
| I3390-6X1L | 04061833856086 |