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About This Item
UNSPSC Code:
12352200
NACRES:
NA.77
Form:
DMSO solution
Solubility:
water: soluble
Storage temp.:
−20°C
Product Name
Protease Inhibitor Cocktail, Animal Component Free, for use with mammalian cell and tissue extract, DMSO solution
Quality Level
form
DMSO solution
solubility
water: soluble
storage temp.
−20°C
General description
Components of this cocktail are of non-animal origin and no animal products were used in the component production process. All vessels and instruments used for the cocktail production are dedicated for animal component free production and have never encountered an animal product. Aprotinin used in this cocktail is a recombinant bovine protein expressed in plants (Nicotiana).
This protease inhibitor cocktail has been optimized and tested for mammalian cell and tissue extracts.
Application
Animal component free (ACF). Optimized and tested for general use. Can also be used for purification of histidine-tagged proteins, as chelating agents have been omitted.
Biochem/physiol Actions
Inhibits serine, cysteine, aspartic proteases, and aminopeptidases.
Physical form
The components in this ACF protease inhibitor cocktail do not originate from animal sources and can thus be used in the production of animal-free proteins.
This ACF cocktail is supplied as a ready-to-use solution in DMSO. One ml of the cocktail solution is recommended for the inhibition of endogenous enzymes found in 100 mL of lysate from 20 g (wet weight) of bovine liver, or 10 mL of cell lysate obtained from CHO cells at a cell density of 108 cells per mL.
Other Notes
AEBSF
Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A
Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A
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Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
Regulatory Information
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| I3786-1ML | 04061833856376 |
| I3786-5ML | 04061833856383 |
