I7633
IMDM
With ʟ-glutamine and HEPES, without sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
Iscove′s MDM, Iscoves MDM, Iscove’s Modified Dulbecco’s Medium, IMDM
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About This Item
Product Name
Iscove′s Modified Dulbecco′s Medium, With L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture
form
powder
technique(s)
cell culture | hybridoma: suitable, cell culture | mammalian: suitable
components
phenol red: yes
glucose: yes
L-glutamine: yes
shipped in
ambient
storage temp.
2-8°C
General description
Iscove′s Modified Dulbecco′s medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids, and vitamins, sodium pyruvate, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer, and potassium nitrate instead of ferric nitrate. Iscove′s medium supports murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.
Application
Iscove′s Modified Dulbecco′s Medium has been used to culture KG1a cells (human leukemic cell line) and Chinese hamster ovary (CHO) DG44 cell line.
Reported to support murine B lymphocytes, hematopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.
Preparation Note
Formulated to contain 17.7 grams of powder per liter of medium.
Supplement with 3.024 g/L sodium bicarbonate.
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Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
粉末培养基及盐混合物极具吸湿性，应注意防潮。
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| I7633-10X1L | 04061833858332 |
| I7633-10L | 04061833858325 |
| I7633-50K | 04061834180449 |
| I7633-50L | 04061833858349 |