K3753
Krebs-Henseleit Buffer Modified
With 2000 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture
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About This Item
UNSPSC Code:
12352207
General description
Krebs-Henseleit Buffer Modified is a balanced salt solution (BSS) that is used in cell culture and plays a role in providing cells with water and inorganic salts for normal cell metabolism. In addition, it provides a buffering system to maintain the optimal pH of the medium and acts as an irrigating, transporting, and diluting agent to maintain the intra- and extra-cellular osmotic balance. BSS provides glucose as an energy source for cell metabolism.
Application
Krebs-Henseleit Buffer Modified has been used:
- to collect the rat brains for ex vivo brain slice experiments
- to measure calcium and sodium in rat pulmonary arterial endothelial cells (PAECs)
- to collect the uterine and forelimb blood vessel samples from horses
Preparation Note
Formulated to contain 9.6 grams of powder per liter of medium.
Supplement with 2.1 g/L sodium bicarbonate.
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
粉末培养基及盐混合物极具吸湿性，应注意防潮。
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
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Cell encapsulation provides a three-dimensional support by incorporating isolated cells into microcapsules with the goal of simultaneously maintaining cell survival and function, as well as providing active transport for a bioreactor in vitro similarly to that observed in vivo. However, the biotra-nsformation
Global Trade Item Number
| SKU | GTIN |
|---|---|
| K3753-10L | 04061842473267 |
| K3753-10X1L | 04053252145780 |
| K3753-25K | 04061834180524 |
| K3753-50K | 04061834180531 |