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Merck
CN

L3019

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)

Isolectin B4 (BSI-B4), lyophilized powder

Synonym(s):

Bandeirea simplicifolia agglutinin, BS-I

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder
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Quality Level

form

lyophilized powder

potency

≤8 μg per mL agglutination activity (using human blood group B erythrocytes), ≥125 μg per mL agglutination activity (using human blood group A erythrocytes)

composition

Protein, ~95% E1%/280

storage temp.

−20°C

Biochem/physiol Actions

BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Physical form

Contains sodium citrate

Analysis Note

Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.
Highly purified.

Other Notes

BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A4 has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

低风险生物材料

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Emil Ruvinov et al.
Fibrogenesis & tissue repair, 1(1), 7-7 (2008-11-19)
Erythropoietin possesses cellular protection properties. The aim of the present study was to test the hypothesis that in situ expression of recombinant human erythropoietin (rhEPO) would improve tissue repair in rat after myocardial infarction (MI). RhEPO-producing cardiac fibroblasts were generated
Ninib Baryawno et al.
Neuro-oncology, 10(5), 661-674 (2008-08-22)
Prostaglandin E(2) (PGE(2)) has been shown to play important roles in several aspects of tumor development and progression. PGE(2) is synthesized from arachidonic acid by cyclooxygenases (COX) and prostaglandin E synthases (PGES) and mediates its biological activity through binding to



Global Trade Item Number

SKUGTIN
L3019-1MG04061826675694