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L3295

Phospholipase A1 from Aspergillus oryzae

Name: Phospholipase A1 from <I>Aspergillus oryzae</I>
Brand: SIGMA

Synonym(s):

Lecitase^™ Ultra, PLA1

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About This Item

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

3.1.1.32 (BRENDA, IUBMB)

MDL number:

MFCD00130786

Specific activity:

≥10 KLU/g

Recombinant:

expressed in Aspergillus oryzae

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Properties

recombinant

expressed in Aspergillus oryzae

Quality Level

200

form

liquid

specific activity

≥10 KLU/g

storage temp.

2-8°C

Description

General description

Phospholipase A1 (PLA1) catalyzes the hydrolysis of acyl group from position 1 of lecithin to yield lysolecithin. It is expressed in a wide range of organisms such as rat platelets, bovine brain and testis, hornet venom, bonito muscle and fungi. Gene coding for PLA1 consists of four exons and three short introns spanning 1,056bp of genomic DNA. Mature protein contains 269 aminoacids and two possible N-glycosylation sites (Asn27 and Asn55).

Application

Phospholipase A1 from Aspergillus oryzae has been used:

in the preparation of sn-1 and sn-2 C18:1- lysophosphatidylcholine (LPC) regioisomer standards
as a catalyst for the synthesis 6-O-glucosyl-poly(3-hydroxyalkanoates) in a micro-aqueous system
to catalyze the synthesis of methyl butanoate and methyl benzoate flavor esters in continuous flow microreactor
to hydrolyze 17:0 phosphocholine (PC)

Analysis Note

minimum activity 10 KLU/G liquid

Legal Information

Lecitase is a trademark of Novozymes Corp.

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Thermomyces lanuginosus lipase-catalyzed synthesis of natural flavor esters in a continuous flow microreactor

Gumel AM and Annuar MSM

3 Biotech, 6(1), 24-24 (2016)

Characterization of secretory phospholipase A₂ with phospholipase A₁ activity in tobacco, Nicotiana tabacum (L.).

Yukichi Fujikawa et al.

Lipids, 47(3), 303-312 (2011-11-30)

A cDNA encoding protein with homology to plant secretory phospholipase A₂ (sPLA₂), denoted as Nt1 PLA₂, was isolated from tobacco (Nicotiana tabacum). The cDNA encodes a mature protein of 118 amino acid residues with a putative signal peptide of 29

Surface loops of extracellular phospholipase A(1) determine both substrate specificity and preference for lysophospholipids.

Naoaki Arima et al.

Journal of lipid research, 53(3), 513-521 (2011-12-17)

Members of the pancreatic lipase family exhibit both lipase activity toward triacylglycerol and/or phospholipase A(1) (PLA(1)) activity toward certain phospholipids. Some members of the pancreatic lipase family exhibit lysophospholipase activity in addition to their lipase and PLA(1) activities. Two such

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Global Trade Item Number

SKU	GTIN
L3295-50ML	04061833951019
L3295-250ML	04061838163943