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L3892

Lectin from Triticum vulgaris (wheat)

Name: Lectin from <I>Triticum vulgaris </I>(wheat)
Brand: SIGMA

peroxidase conjugate, lyophilized powder

Synonym(s):

WGA, Wheat germ agglutinin

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About This Item

NACRES:

NA.32

UNSPSC Code:

12352202

Form:

lyophilized powder

Assay:

≥85% protein basis (Warburg-Christian)

Biological source:

Triticum vulgaris

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Properties

biological source

Triticum vulgaris

Quality Level

200

conjugate

peroxidase conjugate

assay

≥85% protein basis (Warburg-Christian)

form

lyophilized powder

potency

<40 μg/mL agglutination activity

peroxidase activity

50-200 units/mg protein

composition

Protein, ~90% modified Warburg-Christian

storage temp.

−20°C

Description

Application

Detects glycoproteins containing β(1→4)-N-acetyl-D-glucosamine when used with appropriate peroxidase substrate

General Western Blot Protocol:

Glycoprotein sample size: 500ng
Lectin Concentration: 0.1ug/ml

Load samples at 500 ng of glycoprotein per lane
Run 4-20% Bis-Tris SDS page gel
Transfer gel to a PVDF membrane
Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
Wash membrane 5 x 5 minutes with 25ml RIPA buffer
Detect using chemiluminescent substrate (CPS1-120)

Biochem/physiol Actions

WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

Packaging

Package size based on protein content.

Physical form

Contains citrate buffer

Preparation Note

Conjugates are prepared from affinity purified lectin.

Prepared from peroxidase type VI (P8375) using a modification of the method of O′Sullivan, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.

Other Notes

One unit will form 1.0 mg of purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

低风险生物材料

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Global Trade Item Number

SKU	GTIN
L3892-2MG	04061833263310
L3892-1MG	04061826675731
L3892-5MG	04061833263327