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Merck
CN

L3916

L-Lactic Dehydrogenase from bovine heart

1000 units/mL

Synonym(s):

Lactate, (S)-Lactate: NAD+ oxidoreductase, L-LDH, LAD, LD

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About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-617-8
MDL number:
EC Number:
Specific activity:
1000 units/mL
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form

solution

Quality Level

specific activity

1000 units/mL

storage temp.

2-8°C

General description

LDH (lactic dehydrogenase) is a glycolytic enzyme. It is found in skeletal muscle, heart, liver, kidneys, brain, lungs and red blood cells. It comprises of five isoenzyme forms. LDH possess a tetrameric structure.

Application

L-Lactic Dehydrogenase from bovine heart has been used:
  • as a reference standard to measure the LDH (lactic dehydrogenase)
  • to quench protein-bound NADH in cells
  • to measure the lactate released by the cells in suspension

For use in enzymatic determination of lactate or pyruvate.

Biochem/physiol Actions

LDH (lactic dehydrogenase) helps in the reversible conversion of lactate to pyruvate.
Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

Physical form

Suspension in 2.2 M ammonium sulfate

Other Notes

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.


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pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

低风险生物材料

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Protocols

This procedure applies to all products from heart muscle that have a specification for L-Lactic Dehydrogenase activity.

Articles

以硫酸铵悬浮液形式提供的酶的使用指南

Instructions for working with enzymes supplied as ammonium sulfate suspensions


Circulating biomarkers in malignant melanoma
Advances in Clinical Chemistry, 69, 47-89 (2015)
NADH fluorescence lifetime is an endogenous reporter of alpha-synuclein aggregation in live cells
Plotegher N, et al.
Faseb Journal, 29(6), 2484-2494 (2015)
Cardiac, Vascular, and Skeletal Muscle Systems
Haschek and Rousseaux's Handbook of Toxicologic Pathology (2013)



Global Trade Item Number

SKUGTIN
L3916-2.5ML04061835557530