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Merck
CN

L4895

Lectin from Triticum vulgaris (wheat)

FITC conjugate, lyophilized powder

Synonym(s):

WGA, Wheat germ agglutinin

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder
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conjugate

FITC conjugate

Quality Level

form

lyophilized powder

potency

<50 μg/mL agglutination activity

composition

Protein, ~50% Lowry

extent of labeling

~2 mol FITC per mol protein

storage temp.

2-8°C

General description

Lectins are carbohydrate-binding proteins. They are omnipresent and usually found in fungi, plants and animals. The secondary structure of this protein is rich in β-strands and possesses carbohydrate binding sites on the surface.

Application

Lectin from Triticum vulgaris (wheat) has been used:
  • to treat transfected cells for immunofluorescence
  • to stereotaxically inject into mice for neuronal tracing
  • in immunohistochemistry for the analysis of myocytes

For the fluorescent detection of glycoproteins containing β(1→4)-N-acetyl-D-glucosamine

Biochem/physiol Actions

Lectin plays a key role in glycoconjugate characterizing, imaging and targeting. Its use in a microarray assay, enable efficient glycome profiling. In plants and fungi, lectin defends against pathogens/feeders. Lectin also plays a major role in host recognition and tissue adhesion.
WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

Packaging

Package size based on protein content.

Physical form

Contains phosphate buffer salts

Preparation Note

Conjugates are prepared from affinity purified lectin.


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

低风险生物材料

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Articles

Understand sialic acid structure, function, signaling, and modifications. Easily find products for sialic acid research.

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.


Yihua Bei et al.
Journal of cellular and molecular medicine, 21(8), 1648-1655 (2017-03-18)
The adult heart retains a limited ability to regenerate in response to injury. Although exercise can reduce cardiac ischaemia/reperfusion (I/R) injury, the relative contribution of cardiac cell proliferation including newly formed cardiomyocytes remains unclear. A 4-week swimming murine model was
Sarah Aileen Lewis et al.
PloS one, 13(8), e0201986-e0201986 (2018-08-14)
Tissues with high-energy demand including the heart are rich in the energy-producing organelles, mitochondria, and sensitive to mitochondrial dysfunction. While alterations in mitochondrial function are increasingly recognized in cardiovascular diseases, the molecular mechanisms through which changes in mitochondria lead to
Hai Li et al.
Langmuir : the ACS journal of surfaces and colloids, 26(8), 5603-5609 (2009-12-02)
The technique to pattern aminosilanes on hydroxyl-terminated substrates will open up extensive applications in many fields. There are some existing methods to pattern aminosilanes, in particular, (3-aminopropyl)triethoxysilane (APTES) on SiO(2) and glass substrates through indirect routes. However, few reports focus



Global Trade Item Number

SKUGTIN
L4895-10MG04061833263365
L4895-2MG04061833957752
L4895-5MG04061833957769