L5142
Lectin from Triticum vulgaris (wheat)
biotin conjugate, lyophilized powder
Synonym(s):
WGA, Wheat germ agglutinin
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
eCl@ss:
34058011
Form:
lyophilized powder
conjugate
biotin conjugate
Quality Level
form
lyophilized powder
composition
Protein, ≥80% E1%/280
extent of labeling
2-4 mol biotin per mol protein
storage temp.
2-8°C
General description
Wheat germ lectin (WGA) from Triticum vulgaris exists as a homodimer and has multiple N-acetyl glucosamine (GlcNAc) binding sites. It is resistant to protein denaturation agents and is used for the detection of O-GlcNAc proteins. WGA binding to N-glycolylneuraminic acid (Neu5Ac) may modulate pro-inflammatory immune response.
Application
Lectin from Triticum vulgaris (wheat) has been used:
- in lectin binding assay of samples from intrauterine stages of filarial parasites
- in the histological studies of mice myocytes
- for the generation bead-biotin-WGA complex with Dynabeads® M-280 Streptavidin magnetic beads for lectin magnetic separation of Toxoplasma gondii oocytes from water samples
Biochem/physiol Actions
WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.
Packaging
Package size based on protein content.
Physical form
Contains sodium citrate
Preparation Note
Conjugates are prepared from affinity purified lectin.
Legal Information
Dynabeads is a registered trademark of Invitrogen Dynal AS Corp
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
低风险生物材料
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Articles
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WGA-based lectin affinity gel electrophoresis: A novel method for the detection of O-GlcNAc-modified proteins
Kubota Y, et al.
Testing, 12(7), e0180714-e0180714 (2017)
Use of lectin-magnetic separation (LMS) for detecting Toxoplasma gondii oocysts in environmental water samples
Harito JB, et al.
Water Research, 127, 68-76 (2017)
Candida Fagundes Teixeira et al.
PLoS neglected tropical diseases, 1(2), e73-e73 (2007-12-07)
Diagnosis of intestinal schistosomiasis in low endemic areas is a problem because often control measures have reduced egg burdens in feces to below the detection limits of classical coproparasitological methods. Evaluation of molecular methods is hindered by the absence of
Global Trade Item Number
| SKU | GTIN |
|---|---|
| L5142-1MG | 04061833957912 |
| L5142-5MG | 04061833263396 |