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Merck
CN

L5505

Lectin from Ulex europaeus (gorse, furze)

lyophilized powder

Synonym(s):

Ulex europaeus agglutinin, UEA-I

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder
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form

lyophilized powder

Quality Level

potency

<8 μg/mL agglutination activity

composition

Protein, ≥80% biuret

impurities

salt, essentially free

storage temp.

2-8°C

Biochem/physiol Actions

Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.

Preparation Note

Purified by affinity chromatography using the method of Pereira.

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.

Other Notes

Conjugates are prepared from affinity purified lectin.


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

低风险生物材料

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Articles

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.


Immunochemical studies on the combining site of the blood group H-specific lectin 1 from Ulex europeus seeds.
M E Pereira et al.
Archives of biochemistry and biophysics, 185(1), 108-115 (1978-01-15)
R T Sawyer et al.
Infection and immunity, 60(3), 1041-1046 (1992-03-01)
The isolated perfused mouse liver model was used to study the effects of various lectins on hepatic trapping and killing of Candida albicans. After mouse livers were washed with 20 to 30 ml of perfusion buffer, 10(6) C. albicans CFU
M Kristensen et al.
Scandinavian journal of medicine & science in sports, 25 Suppl 2, 40-48 (2015-06-18)
We investigated the effect of alpine skiing for 12 weeks on skeletal muscle characteristics and biomarkers of glucose homeostasis and cardiovascular risk factors. Twenty-three patients with a total knee arthroplasty (TKA) were studied 2.9 ± 0.9 years (mean ± SD) after the operation. Fourteen



Global Trade Item Number

SKUGTIN
L5505-2MG04061833958186
L5505-5MG04061833958193