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About This Item
NACRES:
NA.75
UNSPSC Code:
12352202
Form:
liquid
Technique(s):
cell culture | mammalian: suitable
Impurities:
≤400 EU/mL endotoxin
Concentration:
100 mg/mL BSA in DPBS, 90-120 mg/mL protein (biuret)
description
Contains 2 moles linoleic acid per mole BSA
Quality Level
sterility
sterile-filtered
product line
BioReagent
form
liquid
concentration
100 mg/mL BSA in DPBS, 90-120 mg/mL protein (biuret)
technique(s)
cell culture | mammalian: suitable
impurities
≤400 EU/mL endotoxin
storage temp.
2-8°C
General description
Fatty Acid-Albumin - complexes have been employed as alternative sources of lipids in the development of serum-free media. Fatty acids bind to serum proteins in high proportions. Such proteins may release beneficial fatty acids and bind those that are inhibitory. Linoleic acid, a precursor of prostaglandins bound to BSA is beneficial to the growth of a variety of cell types (e.g., BHK, hybridoma). It also improves its stability and solubility in culture media.
Application
Linoleic Acid-Albumin from bovine serum albumin is used in the cell line culture and cell differentiation medium.
Linoleic Acid-Albumin from bovine serum albumin is used in the cell line culture and cell differentiation medium.
Other Notes
Recommended usage: 1 ml/100 ml medium
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
Regulatory Information
动物来源生物产品
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Articles
Importance and uses of linoleic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures
Related Content
Instructions
Chiu-Fang Liang et al.
IUBMB life, 54(5), 275-279 (2003-02-18)
Epidermal growth factor (EGF) was reported to regulate triacyl glycerol synthesis in various cells. Linoleic acid and its metabolites were thought to modulate the signal transduction of growth factors. This study determined whether linoleic acid regulated the effect of EGF
Dong-Hwan Kim et al.
Lipids, 55(2), 163-171 (2020-02-15)
The regulation of adipocyte differentiation is an important factor for production efficiency and meat quality in the poultry industry. The purpose of this study was to develop a new in vitro model of adipogenic differentiation of chicken embryonic fibroblasts (CEF).
Cécile Coste et al.
PloS one, 12(7), e0177962-e0177962 (2017-07-07)
Adult neural crest stem-derived cells (NCSC) are of extraordinary high plasticity and promising candidates for use in regenerative medicine. Several locations such as skin, adipose tissue, dental pulp or bone marrow have been described in rodent, as sources of NCSC.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| L9530-5ML | 04061833966730 |