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M0643

Minimum Essential Medium Eagle

Name: Minimum Essential Medium Eagle
Brand: SIGMA

With Earle′s salts, L-glutamine, and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

EMEM, MEM

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About This Item

NACRES:

NA.75

UNSPSC Code:

12352207

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Properties

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
Earle’s salts (5% CO₂): yes
NaHCO₃: no
phenol red: yes
L-glutamine: yes
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

Description

General description

It contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser. May be preferred for growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium.

Minimum Essential Medium Eagle consists of inorganic salts, water-soluble vitamins, sugar, and essential amino acids. It is widely used as a media formulation for many cell culture studies. It is a modified version of Basal medium Eagle (BME) and the concentrations of the majority of amino acids are twice as compared to BME.

Application

Minimum Essential Medium Eagle has been used to culture osteoblast-like cell line MG-63, dispersed ventricular cells, and endometrial adenocarcinoma Ishikawa cells.

Preparation Note

Formulated to contain 9.6 grams of powder per liter of medium.

Supplement with 2.2 g/L sodium bicarbonate.

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wgk

WGK 2

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Protocols

粉末培养基制备说明

粉末培养基及盐混合物极具吸湿性，应注意防潮。

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

Articles

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Water for Mammalian Cell Culture

Ensure optimal cell culture outcomes with ultrapure, endotoxin-free water. Learn about the impact of water impurities on mammalian cell cultures and how to achieve optimal cell growth.

Global Trade Item Number

SKU	GTIN
M0643-50KG	04061834408901
M0643-10L	04061833993750
M0643-50K	04061834181491
M0643-25KG	04061834408895
M0643-10X1L	04061835568802
M0643-1L	04061837459504
M0643-50L	04061833993767