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About This Item
NACRES:
NA.75
UNSPSC Code:
12352207
Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
Earle’s salts (5% CO2): yes
L-glutamine: no
HEPES: no
NaHCO3: no
sodium pyruvate: no
phenol red: no
shipped in
ambient
storage temp.
2-8°C
General description
Minimum Essential Medium Eagle contains 19 amino acids. The essential amino acids and the non-essential amino acids: L-ala, L-asn, L-asp, L-glu, L-gly, L-pro, and L-ser. This medium is preferred for the growth of adherent cells in the presence of reduced serum or as a base for the development of a serum-free medium. It is suitable for a wide variety of cells grown in monolayers.
Application
Minimum Essential Medium Eagle has been used:
- to culture endometrial adenocarcinoma Ishikawa cells for bisphenol studies
- to culture MCF-7 cells for experimental treatments with hormone
- to maintain zebrafish pineal glands for action spectrum analysis
Features and Benefits
Contains high concentrations of amino acids so the medium resembles the protein composition of cultured mammalian cells
Preparation Note
Formulated to contain 9.3 grams of powder per liter of medium.
Supplement with 0.292 g/L L-glutamine, 2.2 g/L sodium bicarbonate.
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Storage Class
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
粉末培养基及盐混合物极具吸湿性,应注意防潮。
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
Articles
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| M3024-10L | 04061834046301 |
| M3024-10X1L | 04061834046318 |
| M3024-1L | 04061837473449 |