Skip to Content
Merck
CN

M4642

Minimum Essential Medium Eagle

With Hanks′ salts and ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

EMEM, MEM

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist


Product Name

Minimum Essential Medium Eagle, With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

components

glucose: yes
Hanks’ salts (2% CO2): yes
L-glutamine: yes
Earle’s salts (5% CO2): no
NaHCO3: no
phenol red: yes
sodium pyruvate: no
stable glutamine: no
HEPES: no

shipped in

ambient

storage temp.

2-8°C

General description

Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has high concentrations of amino acids, so the medium closely approximates the protein concentration of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has increased the applicability of this medium. This medium lacks calcium that permits the growth of cells in suspension. Modification of the original MEM formulation. Earle′s salts have been replaced with Hanks′ salts. Since the bicarbonate concentration is substantially lower, this medium has a substantially reduced buffering capacity.

Application

Minimum Essential Medium Eagle (MEM) has been used:

  • as a component of the Hank′s MEM (HMEM)-Pipes buffer to induce anaphylatoxin induced-ear swelling in mice
  • as a component of the slice culture medium (SCM) for the incubation of mouse hippocampal slice cultures
  • to culture Müller cells from guinea pigs

Preparation Note

Formulated to contain 10.6 grams of powder per liter of medium.
Supplement with 0.35 g/L sodium bicarbonate.


Still not finding the right product?

Explore all of our products under Minimum Essential Medium Eagle


Storage Class

11 - Combustible Solids

wgk

WGK 1



Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library


Protocols

粉末培养基及盐混合物极具吸湿性,应注意防潮。

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

Articles

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.


Anthony Dudilot et al.
Current biology : CB, 30(7), 1167-1176 (2020-02-23)
Synaptic plasticity, with its two most studied forms, long-term potentiation (LTP) and long-term depression (LTD), is the cellular mechanism underlying learning and memory. Although it has been known for two decades that bidirectional synaptic plasticity necessitates a corresponding bidirectional regulation
Pasqualino de Antonellis et al.
PloS one, 6(9), e24584-e24584 (2011-09-21)
Through negative regulation of gene expression, microRNAs (miRNAs) can function as oncosuppressors in cancers, and can themselves show altered expression in various tumor types. Here, we have investigated medulloblastoma tumors (MBs), which arise from an early impairment of developmental processes
Carlos Mendoza-Palomares et al.
Eukaryotic cell, 7(4), 684-697 (2008-02-19)
Cysteine proteases have been shown to be essential virulence factors and drug targets in trypanosomatids and an attractive antidisease vaccine candidate for Trypanosoma congolense. Here, we describe an important amplification of genes encoding cathepsin B-like proteases unique to T. congolense.



Global Trade Item Number

SKUGTIN
M4642-50L04061834059103
M4642-25KG04061834409069
M4642-50KG04061834409076
M4642-10L04061834059097
M4642-50K04061834171935